Linalool elicits vasorelaxation of mouse aortae through activation of guanylyl cyclase and K<sup>+</sup> channels

Purum Kang, Geun Hee Seol

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objectives The aim of this study was to investigate the cardiovascular relaxing properties of monoterpene alcohol (-)-linalool (LIN), a principal component of several aromatic plants. Methods We assessed the effects of LIN on vascular contractility in mouse aortae and evaluated its underlying mechanisms of action. Key findings We found that LIN dose-dependently relaxed the vascular tonus of mouse thoracic aortae induced by prostaglandin F<inf>2</inf> alpha (PGF<inf>2α</inf>, 3 μm). This effect, however, was reduced by pretreatment with the nitric oxide synthase inhibitor L-NAME (30 μm). Treatment with the inhibitor of soluble guanylyl cyclase ODQ (2 μm) or the K<sup>+</sup> channel blocker TEA (10 mM) partially blocked LIN-induced vasorelaxation. Moreover, addition of TEA after incubation of the rings with L-NAME and ODQ partially blocked LIN-induced vasorelaxation. Furthermore, LIN was able to partially antagonize CaCl<inf>2</inf>-induced contractions in high potassium (80 mM) Krebs' solution, whereas LIN did not affect Ca<sup>2+</sup> release from endoplasmic reticulum Ca<sup>2+</sup> stores. Conclusion Our findings indicate that LIN may induce endothelium-dependent vasorelaxation in mouse thoracic aortae by activating soluble guanylyl cyclase and K<sup>+</sup> channels.

Original languageEnglish
Pages (from-to)714-719
Number of pages6
JournalJournal of Pharmacy and Pharmacology
Volume67
Issue number5
DOIs
Publication statusPublished - 2015 May 1

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Guanylate Cyclase
Vasodilation
Aorta
Dinoprost
NG-Nitroarginine Methyl Ester
Thoracic Aorta
Blood Vessels
Monoterpenes
linalool
Nitric Oxide Synthase
Endoplasmic Reticulum
Endothelium
Potassium
Alcohols

Keywords

  • (-)-linalool
  • K<sup>+</sup> channel
  • soluble guanylyl cyclase
  • vasorelaxation

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

Linalool elicits vasorelaxation of mouse aortae through activation of guanylyl cyclase and K<sup>+</sup> channels. / Kang, Purum; Seol, Geun Hee.

In: Journal of Pharmacy and Pharmacology, Vol. 67, No. 5, 01.05.2015, p. 714-719.

Research output: Contribution to journalArticle

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abstract = "Objectives The aim of this study was to investigate the cardiovascular relaxing properties of monoterpene alcohol (-)-linalool (LIN), a principal component of several aromatic plants. Methods We assessed the effects of LIN on vascular contractility in mouse aortae and evaluated its underlying mechanisms of action. Key findings We found that LIN dose-dependently relaxed the vascular tonus of mouse thoracic aortae induced by prostaglandin F2 alpha (PGF2α, 3 μm). This effect, however, was reduced by pretreatment with the nitric oxide synthase inhibitor L-NAME (30 μm). Treatment with the inhibitor of soluble guanylyl cyclase ODQ (2 μm) or the K+ channel blocker TEA (10 mM) partially blocked LIN-induced vasorelaxation. Moreover, addition of TEA after incubation of the rings with L-NAME and ODQ partially blocked LIN-induced vasorelaxation. Furthermore, LIN was able to partially antagonize CaCl2-induced contractions in high potassium (80 mM) Krebs' solution, whereas LIN did not affect Ca2+ release from endoplasmic reticulum Ca2+ stores. Conclusion Our findings indicate that LIN may induce endothelium-dependent vasorelaxation in mouse thoracic aortae by activating soluble guanylyl cyclase and K+ channels.",
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N2 - Objectives The aim of this study was to investigate the cardiovascular relaxing properties of monoterpene alcohol (-)-linalool (LIN), a principal component of several aromatic plants. Methods We assessed the effects of LIN on vascular contractility in mouse aortae and evaluated its underlying mechanisms of action. Key findings We found that LIN dose-dependently relaxed the vascular tonus of mouse thoracic aortae induced by prostaglandin F2 alpha (PGF2α, 3 μm). This effect, however, was reduced by pretreatment with the nitric oxide synthase inhibitor L-NAME (30 μm). Treatment with the inhibitor of soluble guanylyl cyclase ODQ (2 μm) or the K+ channel blocker TEA (10 mM) partially blocked LIN-induced vasorelaxation. Moreover, addition of TEA after incubation of the rings with L-NAME and ODQ partially blocked LIN-induced vasorelaxation. Furthermore, LIN was able to partially antagonize CaCl2-induced contractions in high potassium (80 mM) Krebs' solution, whereas LIN did not affect Ca2+ release from endoplasmic reticulum Ca2+ stores. Conclusion Our findings indicate that LIN may induce endothelium-dependent vasorelaxation in mouse thoracic aortae by activating soluble guanylyl cyclase and K+ channels.

AB - Objectives The aim of this study was to investigate the cardiovascular relaxing properties of monoterpene alcohol (-)-linalool (LIN), a principal component of several aromatic plants. Methods We assessed the effects of LIN on vascular contractility in mouse aortae and evaluated its underlying mechanisms of action. Key findings We found that LIN dose-dependently relaxed the vascular tonus of mouse thoracic aortae induced by prostaglandin F2 alpha (PGF2α, 3 μm). This effect, however, was reduced by pretreatment with the nitric oxide synthase inhibitor L-NAME (30 μm). Treatment with the inhibitor of soluble guanylyl cyclase ODQ (2 μm) or the K+ channel blocker TEA (10 mM) partially blocked LIN-induced vasorelaxation. Moreover, addition of TEA after incubation of the rings with L-NAME and ODQ partially blocked LIN-induced vasorelaxation. Furthermore, LIN was able to partially antagonize CaCl2-induced contractions in high potassium (80 mM) Krebs' solution, whereas LIN did not affect Ca2+ release from endoplasmic reticulum Ca2+ stores. Conclusion Our findings indicate that LIN may induce endothelium-dependent vasorelaxation in mouse thoracic aortae by activating soluble guanylyl cyclase and K+ channels.

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