TY - JOUR
T1 - Linking multiple biomarker responses in Daphnia magna under thermal stress
AU - Samanta, Palas
AU - Im, Hyungjoon
AU - Shim, Taeyong
AU - Na, Joorim
AU - Jung, Jinho
N1 - Funding Information:
This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korea government ( NRF-2016R1A2B4016299 and NRF-2019R1A2C1002890 ).
Publisher Copyright:
© 2020 Elsevier Ltd
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/8
Y1 - 2020/8
N2 - Temperature is an important abiotic variable that greatly influences the performance of aquatic ectotherms, especially under current anthropogenic global warming and thermal discharges. The aim of the present study was to evaluate thermal stress (20 °C vs 28 °C) in Daphnia magna over 21 d, focusing on the linkage among molecular and biochemical biomarker responses. Thermal stress significantly increased the levels of reactive oxygen species (ROS) and lipid peroxidation, especially in the 3-d short-term exposure treatment. This change in the ROS level was also correlated with mitochondrial membrane damage. These findings suggest that oxidative stress is the major pathway for thermally-induced toxicity of D. magna. Additionally, the expression levels of genes related to hypoxia (Hb), development (Vtg1), and sex determination (Dsx1-α, Dsx1-β, and Dsx2) were greatly increased by elevated temperature in a time-dependent manner. The cellular energy allocation was markedly decreased at the elevated temperature in the 3-d exposure treatment, mainly due to carbohydrates consumption for survival (oxidative stress defense). The present study showed that linking multiples biomarker responses are crucial for understanding the underlying mechanism of thermal stress on D. magna.
AB - Temperature is an important abiotic variable that greatly influences the performance of aquatic ectotherms, especially under current anthropogenic global warming and thermal discharges. The aim of the present study was to evaluate thermal stress (20 °C vs 28 °C) in Daphnia magna over 21 d, focusing on the linkage among molecular and biochemical biomarker responses. Thermal stress significantly increased the levels of reactive oxygen species (ROS) and lipid peroxidation, especially in the 3-d short-term exposure treatment. This change in the ROS level was also correlated with mitochondrial membrane damage. These findings suggest that oxidative stress is the major pathway for thermally-induced toxicity of D. magna. Additionally, the expression levels of genes related to hypoxia (Hb), development (Vtg1), and sex determination (Dsx1-α, Dsx1-β, and Dsx2) were greatly increased by elevated temperature in a time-dependent manner. The cellular energy allocation was markedly decreased at the elevated temperature in the 3-d exposure treatment, mainly due to carbohydrates consumption for survival (oxidative stress defense). The present study showed that linking multiples biomarker responses are crucial for understanding the underlying mechanism of thermal stress on D. magna.
KW - Biomarker
KW - Daphnid
KW - Mitotoxicity
KW - Oxidative stress
KW - Thermal stress
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U2 - 10.1016/j.envpol.2020.114432
DO - 10.1016/j.envpol.2020.114432
M3 - Article
C2 - 32247115
AN - SCOPUS:85082511807
VL - 263
JO - Environmental Pollution
JF - Environmental Pollution
SN - 0269-7491
M1 - 114432
ER -