Lipase-catalyzed acidolysis of perilla oil with caprylic acid to produce structured lipids

In-Hwan Kim, Hakryul Kim, Ki Teak Lee, Soo Hyun Chung, Soon N. Ko

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Structured lipids were synthesized by acidolysis of perilla oil and caprylic acid using two lipases, Lipozyme RM IM from Rhizomucor miehei and Lipozyme TL IM from Thermomyces lanuginosa. Effects of molar ratio, reaction time, reaction temperature, enzyme load, and solvent content on acidolysis reactions were studied. The solvent content ranged from 0.0 (solvent-free) to 85.3%. The results showed that the incorporation increased in parallel with solvent content to 49.0% with Lipozyme RM IM and to 63.8% with Lipozyme TL IM. After 24 h incubation in n-hexane, caprylic acids were incorporated to 48.5 mol% with Lipozyme RM IM and to 51.4 mol% with Lipozyme TL IM, respectively, whereas linolenic acid content was reduced from 61.4 to 31.5 mol% with Lipozyme RM IM and to 28.4 mol% with Lipozyme TL IM, respectively. Lipozyme TL IM showed a higher acyl migration rate than Lipozyme RM IM when acidolysis was performed in the reaction system containing n-hexane as a solvent, whereas the difference in acyl migration between the two lipases in the solvent-free system was negligible.

Original languageEnglish
Pages (from-to)363-367
Number of pages5
JournalJAOCS, Journal of the American Oil Chemists' Society
Volume79
Issue number4
Publication statusPublished - 2002 Apr 1

Fingerprint

acidolysis
structured lipids
octanoic acid
Lipases
Lipase
Lipids
Acids
Hexane
hexane
Thermomyces
Rhizomucor miehei
linolenic acid
Caprylates
Rhizomucor
perilla oil
Oils
Lipozyme
perilla seed oil
Enzymes
alpha-Linolenic Acid

Keywords

  • Acidolysis
  • Acyl migration
  • Caprylic acid
  • Lipase
  • Perilla oil
  • Structured lipids

ASJC Scopus subject areas

  • Food Science
  • Chemistry (miscellaneous)

Cite this

Lipase-catalyzed acidolysis of perilla oil with caprylic acid to produce structured lipids. / Kim, In-Hwan; Kim, Hakryul; Lee, Ki Teak; Chung, Soo Hyun; Ko, Soon N.

In: JAOCS, Journal of the American Oil Chemists' Society, Vol. 79, No. 4, 01.04.2002, p. 363-367.

Research output: Contribution to journalArticle

Kim, In-Hwan ; Kim, Hakryul ; Lee, Ki Teak ; Chung, Soo Hyun ; Ko, Soon N. / Lipase-catalyzed acidolysis of perilla oil with caprylic acid to produce structured lipids. In: JAOCS, Journal of the American Oil Chemists' Society. 2002 ; Vol. 79, No. 4. pp. 363-367.
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