Lipase-Catalyzed Production of Pinolenic Acid Concentrate from Pine Nut Oil Using a Recirculating Packed Bed Reactor

Tingting Zhao, Byung Hee Kim, Seung In Hong, Sung Won Yoon, Chong Tai Kim, Yangha Kim, In-Hwan Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Pinolenic acid (PLA) concentrate in fatty acid ethyl ester (FAEE) was efficiently produced from pine nut oil via lipase-catalyzed ethanolysis using a recirculating packed bed reactor (RPBR). The effects of reaction temperature, molar ratio, and residence time on the concentration of PLA were explored. Novozym 435 lipase from Candida antarctica showed less selectivity toward PLA esterified at the sn-3 position when temperature was increased from 45 to 55°C. For the trials of molar ratio between 1: 50 and 1: 100 (pine nut oil to ethanol), there were no significant differences in the yield of PLA. Residence time of substrate in a RPBR affected significantly the PLA content as well as the yield of PLA. Optimal temperature, molar ratio (pine nut oil to ethanol), and residence time for production of PLA concentrate via lipase-catalyzed ethanolysis in a RPBR were 45°C, 1: 50, and 3 min, respectively. Under these conditions, the maximal PLA content (36.1 mol%) in the concentrate was obtained during the initial 10 min of reaction.

Original languageEnglish
JournalJournal of Food Science
Volume77
Issue number2
DOIs
Publication statusPublished - 2012 Feb 1

Fingerprint

nut oils
Nuts
Lipase
Oils
concentrates
Pinus
acids
ethanolysis
Temperature
Ethanol
ethanol
Pseudozyma antarctica
temperature
5,9,12-octadecatrienoic acid
Candida
Esters
Fatty Acids
esters
fatty acids

Keywords

  • Candida antarctica
  • Ethanolysis
  • Pine nut oil
  • Pinolenic acid (PLA)
  • Recirculating packed bed reactor (RPBR)

ASJC Scopus subject areas

  • Food Science

Cite this

Lipase-Catalyzed Production of Pinolenic Acid Concentrate from Pine Nut Oil Using a Recirculating Packed Bed Reactor. / Zhao, Tingting; Kim, Byung Hee; Hong, Seung In; Yoon, Sung Won; Kim, Chong Tai; Kim, Yangha; Kim, In-Hwan.

In: Journal of Food Science, Vol. 77, No. 2, 01.02.2012.

Research output: Contribution to journalArticle

Zhao, Tingting ; Kim, Byung Hee ; Hong, Seung In ; Yoon, Sung Won ; Kim, Chong Tai ; Kim, Yangha ; Kim, In-Hwan. / Lipase-Catalyzed Production of Pinolenic Acid Concentrate from Pine Nut Oil Using a Recirculating Packed Bed Reactor. In: Journal of Food Science. 2012 ; Vol. 77, No. 2.
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