Lipase-catalyzed synthesis of 2-ethylhexyl palmitate in a solvent free system using step changes in temperature

Suhyeon Choi, Byung Hee Kim, Da Som No, Sung Won Yoon, Moon Won Lee, Dong Joong Im, In Hwan Kim

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Lipase-catalyzed synthesis of 2-ethylhexyl palmitate (2-EHP) from 2-ethylhexyl alcohol and palmitic acid was carried out in a solvent-free system. A commercial liquid lipase (Eversa Transform 2.0, Novozymes) from Thermomyces lanuginosus was immobilized on Lewatit VP OC 1600, a macroporous hydrophobic carrier. The efficacy of this Eversa immobilized lipase prepared in this study was evaluated on the synthesis of 2-EHP compared with that of Novozym 435 (from Candida antarctica), Lipozyme RM IM (from Rhizomucor miehei), Lipozyme TL IM (from Thermomyces lanuginosus), and the liquid Eversa lipase (commercial name: Eversa Transform 2.0). Among these lipases, the Eversa immobilized lipase was the most effective for the synthesis of 2-EHP. Optimum conditions for the synthesis of 2-EHP using this enzyme were a temperature of 55 °C and enzyme loading of 2% (based on the total weight of substrate). The conversion of 97% was achieved under these optimum conditions. Finally, as a cost-saving strategy, a step change in the reaction temperature was introduced. When a step change in the reaction temperature between 55 °C and 45 °C used, an identical degree of conversion was achieved compared to a constant reaction temperature of 55 °C throughout the reaction.

Original languageEnglish
Article number108261
JournalBiochemical Engineering Journal
Volume177
DOIs
Publication statusPublished - 2022 Jan

Keywords

  • 2-Ethylhexyl palmitate
  • Eversa immobilized lipase
  • Hydrophobic carrier
  • Step change
  • Temperature

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Environmental Engineering
  • Biomedical Engineering

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