Lipase-Mediated Synthesis of Fatty Acid Esters Using a Blending Alcohol Consisting of Methanol and 1-Butanol

Ting Ting Zhao, Nakyung Choi, Heejin Kim, Yangha Kim, Hak Ryul Kim, In-Hwan Kim

Research output: Contribution to journalArticle

Abstract

Lipase-mediated transesterification of soybean oil with a blending alcohol consisting of methanol and 1-butanol for synthesis of fatty acid esters was carried out. Lipase from Thermomyces lanuginosa (Lipozyme TL IM) was used as a biocatalyst. The lipase was purchased from Novozymes (Seoul, Republic of Korea). The effects of the molar proportions of methanol and 1-butanol in the blending alcohol, reaction temperature, enzyme loading and water content were investigated, for reaction optimization. The relative consuming rates of methanol and 1-butanol during the reaction were also explored. Among seven different ratios of alcohol blends employed in this study, that containing 80 mol% methanol gave the highest yield of fatty acid esters. Optimum reaction temperature, enzyme loading, and water content were 30 °C, 15% (based on the substrate weight), and 0.3% (based on the substrate weight), respectively. Water influenced significantly the reaction rate and yield. On the transesterification, the degree of reaction of methanol was higher than that of 1-butanol and the presence of 1-butanol contributed to increase of the reaction rate as well as yield. The maximum yield of ca. 98 wt% was achieved under the optimized condition.

Original languageEnglish
Pages (from-to)559-565
Number of pages7
JournalJAOCS, Journal of the American Oil Chemists' Society
Volume94
Issue number4
DOIs
Publication statusPublished - 2017 Apr 1

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1-Butanol
Lipases
Lipase
Butenes
Fatty acids
Methanol
Esters
Alcohols
Fatty Acids
Transesterification
Water content
Reaction rates
Enzymes
Soybean oil
Soybean Oil
Biocatalysts
Substrates
Temperature
Water

Keywords

  • 1-Butanol
  • Blending alcohol
  • Fatty acid esters
  • Lipase
  • Methanol
  • Transesterification

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Organic Chemistry

Cite this

Lipase-Mediated Synthesis of Fatty Acid Esters Using a Blending Alcohol Consisting of Methanol and 1-Butanol. / Zhao, Ting Ting; Choi, Nakyung; Kim, Heejin; Kim, Yangha; Kim, Hak Ryul; Kim, In-Hwan.

In: JAOCS, Journal of the American Oil Chemists' Society, Vol. 94, No. 4, 01.04.2017, p. 559-565.

Research output: Contribution to journalArticle

Zhao, Ting Ting ; Choi, Nakyung ; Kim, Heejin ; Kim, Yangha ; Kim, Hak Ryul ; Kim, In-Hwan. / Lipase-Mediated Synthesis of Fatty Acid Esters Using a Blending Alcohol Consisting of Methanol and 1-Butanol. In: JAOCS, Journal of the American Oil Chemists' Society. 2017 ; Vol. 94, No. 4. pp. 559-565.
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