Lipid raft proteome reveals that oxidative phosphorylation system is associated with the plasma membrane

Bong Woo Kim, Chang Seok Lee, Jae Sung Yi, Joo Hyung Lee, Joong Won Lee, Hyo Jung Choo, Soon Young Jung, Min Sik Kim, Sang Won Lee, Myung Shik Lee, Gyesoon Yoon, Young-Gyu Ko

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Although accumulating proteomic analyses have supported the fact that mitochondrial oxidative phosphorylation (OXPHOS) complexes are localized in lipid rafts, which mediate cell signaling, immune response and host-pathogen interactions, there has been no in-depth study of the physiological functions of lipid-raft OXPHOS complexes. Here, we show that many subunits of OXPHOS complexes were identified from the lipid rafts of human adipocytes, C2C12 myotubes, Jurkat cells and surface biotin-labeled Jurkat cells via shotgun proteomic analysis. We discuss the findings of OXPHOS complexes in lipid rafts, the role of the surface ATP synthase complex as a receptor for various ligands and extracellular superoxide generation by plasma membrane oxidative phosphorylation complexes.

Original languageEnglish
Pages (from-to)849-866
Number of pages18
JournalExpert Review of Proteomics
Volume7
Issue number6
DOIs
Publication statusPublished - 2010 Dec 1

Fingerprint

Oxidative Phosphorylation
Proteome
Cell membranes
Cell Membrane
Lipids
Jurkat Cells
Proteomics
Cell signaling
Host-Pathogen Interactions
Skeletal Muscle Fibers
Firearms
Pathogens
Biotin
Adipocytes
Superoxides
Adenosine Triphosphate
Ligands

Keywords

  • ATP synthase
  • detergent-resistant lipid rafts
  • extracellular ATP
  • extracellular superoxide
  • oxidative phosphorylation complexes
  • oxygen consumption

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Lipid raft proteome reveals that oxidative phosphorylation system is associated with the plasma membrane. / Kim, Bong Woo; Lee, Chang Seok; Yi, Jae Sung; Lee, Joo Hyung; Lee, Joong Won; Choo, Hyo Jung; Jung, Soon Young; Kim, Min Sik; Lee, Sang Won; Lee, Myung Shik; Yoon, Gyesoon; Ko, Young-Gyu.

In: Expert Review of Proteomics, Vol. 7, No. 6, 01.12.2010, p. 849-866.

Research output: Contribution to journalArticle

Kim, BW, Lee, CS, Yi, JS, Lee, JH, Lee, JW, Choo, HJ, Jung, SY, Kim, MS, Lee, SW, Lee, MS, Yoon, G & Ko, Y-G 2010, 'Lipid raft proteome reveals that oxidative phosphorylation system is associated with the plasma membrane', Expert Review of Proteomics, vol. 7, no. 6, pp. 849-866. https://doi.org/10.1586/epr.10.87
Kim, Bong Woo ; Lee, Chang Seok ; Yi, Jae Sung ; Lee, Joo Hyung ; Lee, Joong Won ; Choo, Hyo Jung ; Jung, Soon Young ; Kim, Min Sik ; Lee, Sang Won ; Lee, Myung Shik ; Yoon, Gyesoon ; Ko, Young-Gyu. / Lipid raft proteome reveals that oxidative phosphorylation system is associated with the plasma membrane. In: Expert Review of Proteomics. 2010 ; Vol. 7, No. 6. pp. 849-866.
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