Lipolytic efficacy of alginate double-layer nanoemulsion containing oleoresin capsicum in differentiated 3T3-L1 adipocytes

Mak Soon Lee, Sunyoon Jung, Yoonjin Shin, Seohyun Lee, Chong Tai Kim, In Hwan Kim, Yangha Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Oleoresin capsicum (OC) is an organic extract from fruits of the genus Capsicum, and has been reported to have an anti-obesity effect. Objective: This study comparatively investigated lipolytic effects of single-layer nanoemulsion (SN) and alginate double-layer nanoemulsion (AN) containing OC in 3T3-L1 adipocytes. Methods: SN and AN were compared by analyzing the intracellular lipid accumulation, triglyceride (TG) content, release of free fatty acids (FFAs) and glycerol, and mRNA expression of genes related to adipogenesis and lipolysis were analyzed in fully differentiated 3T3-L1 adipocytes. Results: Compared with SN, AN exhibited higher efficiency in inhibiting the intracellular lipid accumulation and TG content, and enhanced the release of FFAs and glycerol into the medium. In AN-treated cells, mRNA levels of peroxisome proliferator-activated receptor-γ and the fatty acid-binding protein adipocyte protein-2, which are involved in adipogenesis, were down-regulated, whereas those of genes related to lipolysis, including hormone-sensitive lipase and carnitine palmitoyl transferase-1α, were up-regulated compared with SN-treated cells. Conclusion: The lipolytic effect of AN was greater than that of SN; this was partly associated with the increased TG hydrolysis via induction of lipolytic gene expression and suppression of adipogenic gene expression in 3T3-L1 adipocytes.

Original languageEnglish
Article number1339553
JournalFood and Nutrition Research
Volume61
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

nanoemulsions
oleoresins
Capsicum
alginates
adipocytes
Adipocytes
Adipogenesis
Triglycerides
Lipolysis
Gene Expression
Nonesterified Fatty Acids
Glycerol
triacylglycerols
lipolysis
Sterol Esterase
Lipids
Fatty Acid-Binding Proteins
gene expression
Messenger RNA
Peroxisome Proliferator-Activated Receptors

Keywords

  • Free fatty acid
  • Glycerol
  • Lipolysis
  • MRNA expression
  • Obesity

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics
  • Public Health, Environmental and Occupational Health

Cite this

Lipolytic efficacy of alginate double-layer nanoemulsion containing oleoresin capsicum in differentiated 3T3-L1 adipocytes. / Lee, Mak Soon; Jung, Sunyoon; Shin, Yoonjin; Lee, Seohyun; Kim, Chong Tai; Kim, In Hwan; Kim, Yangha.

In: Food and Nutrition Research, Vol. 61, 1339553, 01.01.2017.

Research output: Contribution to journalArticle

Lee, Mak Soon ; Jung, Sunyoon ; Shin, Yoonjin ; Lee, Seohyun ; Kim, Chong Tai ; Kim, In Hwan ; Kim, Yangha. / Lipolytic efficacy of alginate double-layer nanoemulsion containing oleoresin capsicum in differentiated 3T3-L1 adipocytes. In: Food and Nutrition Research. 2017 ; Vol. 61.
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