TY - JOUR
T1 - Lithospermum erythrorhizon sieb. et zucc. suppresses 3-hydroxy-3-methyl-glutaryl-coa reductase and induces ldl receptor expression in HepG2 cells
AU - Jun, Hee Jin
AU - Jeun, Jungae
AU - Kim, Sang Yeon
AU - Choi, Dal Woong
AU - Kim, Ji Young
AU - Kim, Sung Hoon
AU - Lee, Sung Joon
PY - 2011/6
Y1 - 2011/6
N2 - We examined the effects of Lithospermum erythrorhizon Sieb. et Zucc. (LE) on cholesterol metabolism in vitro. The ethanolic LE extract (ELE) had total polyphenolic and flavonoid contents of 353±7 and 285±51mg/dL, respectively. The ELE inhibited Cu2+-mediated LDL oxidation at <400μg/mL. In HepG2 cells treated with 400μg/mL ELE, the expression of 3-hydroxy-3-methyl-glutaryl-CoA reductase decreased markedly (45%; P<0.05), whereas that of the LDL receptor increased (230%; P<0.05). The protein levels of both were altered similarly. The ELE also increased membrane-bound and cell-associated LDL particles, possibly via upregulation of the LDL receptor. In hepatocytes, 400μg/mL ELE affected surrogate markers of HDL and LDL synthesis: significant expression of apolipoprotein A-I was induced (20%; P<0.05), whereas that of apoB was suppressed (30%; P<0.05). In conclusion, ELE may improve cellular cholesterol metabolism by inhibiting cholesterol biosynthesis and apoB production, accelerating plasma LDL uptake and reducing LDL oxidation.
AB - We examined the effects of Lithospermum erythrorhizon Sieb. et Zucc. (LE) on cholesterol metabolism in vitro. The ethanolic LE extract (ELE) had total polyphenolic and flavonoid contents of 353±7 and 285±51mg/dL, respectively. The ELE inhibited Cu2+-mediated LDL oxidation at <400μg/mL. In HepG2 cells treated with 400μg/mL ELE, the expression of 3-hydroxy-3-methyl-glutaryl-CoA reductase decreased markedly (45%; P<0.05), whereas that of the LDL receptor increased (230%; P<0.05). The protein levels of both were altered similarly. The ELE also increased membrane-bound and cell-associated LDL particles, possibly via upregulation of the LDL receptor. In hepatocytes, 400μg/mL ELE affected surrogate markers of HDL and LDL synthesis: significant expression of apolipoprotein A-I was induced (20%; P<0.05), whereas that of apoB was suppressed (30%; P<0.05). In conclusion, ELE may improve cellular cholesterol metabolism by inhibiting cholesterol biosynthesis and apoB production, accelerating plasma LDL uptake and reducing LDL oxidation.
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U2 - 10.1111/j.1745-4514.2010.00500.x
DO - 10.1111/j.1745-4514.2010.00500.x
M3 - Article
AN - SCOPUS:79958210497
VL - 35
SP - 997
EP - 1013
JO - Journal of Food Biochemistry
JF - Journal of Food Biochemistry
SN - 0145-8884
IS - 3
ER -