Live-cell imaging and profiling of c-Jun N-terminal kinases using covalent inhibitor-derived probes

Linghui Qian; Sijun Pan; Jun Seok Lee; Jingyan Ge; Lin Li; Shao Q. Yao

doi:10.1039/c8cc09558b

Live-cell imaging and profiling of c-Jun N-terminal kinases using covalent inhibitor-derived probes

Linghui Qian, Sijun Pan, Jun Seok Lee, Jingyan Ge, Lin Li, Shao Q. Yao

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

c-Jun N-terminal kinases (JNKs) are involved in critical cellular functions. Herein, small-molecule JNK-targeting probes are reported based on a covalent inhibitor. Together with newly developed two-photon fluorescence Turn-ON reporters and chemoproteomic studies, we showed that some probes may be suitable for live-cell imaging and profiling of JNKs.

Original language	English
Pages (from-to)	1092-1095
Number of pages	4
Journal	Chemical Communications
Volume	55
Issue number	8
DOIs	https://doi.org/10.1039/c8cc09558b
Publication status	Published - 2019
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c8cc09558b

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title = "Live-cell imaging and profiling of c-Jun N-terminal kinases using covalent inhibitor-derived probes",

abstract = "c-Jun N-terminal kinases (JNKs) are involved in critical cellular functions. Herein, small-molecule JNK-targeting probes are reported based on a covalent inhibitor. Together with newly developed two-photon fluorescence Turn-ON reporters and chemoproteomic studies, we showed that some probes may be suitable for live-cell imaging and profiling of JNKs.",

author = "Linghui Qian and Sijun Pan and Lee, {Jun Seok} and Jingyan Ge and Lin Li and Yao, {Shao Q.}",

note = "Funding Information: We thank Dr Hailong Zhang (NUS) for his help with chemical synthesis. Financial support was provided by the Synthetic Biology Research & Development Programme (SBP) of National Research Foundation (NRF), the GSK-EDB Trust Fund (R-143-000-688-592), MOE-T1 (R-143-000-694-114) of Singapore, the National Natural Science Foundation of China (21877100, 21708034, 81672508 and 61505076) and the Jiangsu Provincial Foundation for Distinguished Young Scholars (BK20170041). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",

year = "2019",

doi = "10.1039/c8cc09558b",

language = "English",

volume = "55",

pages = "1092--1095",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "8",

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TY - JOUR

T1 - Live-cell imaging and profiling of c-Jun N-terminal kinases using covalent inhibitor-derived probes

AU - Qian, Linghui

AU - Pan, Sijun

AU - Lee, Jun Seok

AU - Ge, Jingyan

AU - Li, Lin

AU - Yao, Shao Q.

N1 - Funding Information: We thank Dr Hailong Zhang (NUS) for his help with chemical synthesis. Financial support was provided by the Synthetic Biology Research & Development Programme (SBP) of National Research Foundation (NRF), the GSK-EDB Trust Fund (R-143-000-688-592), MOE-T1 (R-143-000-694-114) of Singapore, the National Natural Science Foundation of China (21877100, 21708034, 81672508 and 61505076) and the Jiangsu Provincial Foundation for Distinguished Young Scholars (BK20170041). Publisher Copyright: © The Royal Society of Chemistry.

PY - 2019

Y1 - 2019

N2 - c-Jun N-terminal kinases (JNKs) are involved in critical cellular functions. Herein, small-molecule JNK-targeting probes are reported based on a covalent inhibitor. Together with newly developed two-photon fluorescence Turn-ON reporters and chemoproteomic studies, we showed that some probes may be suitable for live-cell imaging and profiling of JNKs.

AB - c-Jun N-terminal kinases (JNKs) are involved in critical cellular functions. Herein, small-molecule JNK-targeting probes are reported based on a covalent inhibitor. Together with newly developed two-photon fluorescence Turn-ON reporters and chemoproteomic studies, we showed that some probes may be suitable for live-cell imaging and profiling of JNKs.

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U2 - 10.1039/c8cc09558b

DO - 10.1039/c8cc09558b

M3 - Article

C2 - 30620026

AN - SCOPUS:85060237671

VL - 55

SP - 1092

EP - 1095

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 8

ER -

Live-cell imaging and profiling of c-Jun N-terminal kinases using covalent inhibitor-derived probes

Abstract

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