Live cell refractometry using hilbert phase microscopy and confocal reflectance microscopy

Niyom Lue, Wonshik Choi, Gabriel Popescu, Zahid Yaqoob, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Research output: Contribution to journalArticle

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Abstract

Quantitative chemical analysis has served as a useful tool for understanding cellular metabolisms in biology. Among many physical properties used in chemical analysis, refractive index in particular has provided molecular concentration that is an important indicator for biological activities. In this report, we present a method of extracting full-field refractive index maps of live cells in their native states. We first record full-field optical thickness maps of living cells by Hilbert phase microscopy and then acquire physical thickness maps of the same cells using a custom-built confocal reflectance microscope. Full-field and axially averaged refractive index maps are acquired from the ratio of optical thickness to physical thickness. The accuracy of the axially averaged index measurement is 0.002. This approach can provide novel biological assays of label-free living cells in situ.

Original languageEnglish
Pages (from-to)13327-13330
Number of pages4
JournalJournal of Physical Chemistry A
Volume113
Issue number47
DOIs
Publication statusPublished - 2009 Dec 1

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Refractometry
Confocal Microscopy
Microscopy
Microscopic examination
microscopy
reflectance
Refractive index
refractivity
cells
chemical analysis
optical thickness
Cells
metabolism
activity (biology)
Bioactivity
Chemical analysis
biology
Metabolism
Biological Assay
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ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Lue, N., Choi, W., Popescu, G., Yaqoob, Z., Badizadegan, K., Dasari, R. R., & Feld, M. S. (2009). Live cell refractometry using hilbert phase microscopy and confocal reflectance microscopy. Journal of Physical Chemistry A, 113(47), 13327-13330. https://doi.org/10.1021/jp904746r

Live cell refractometry using hilbert phase microscopy and confocal reflectance microscopy. / Lue, Niyom; Choi, Wonshik; Popescu, Gabriel; Yaqoob, Zahid; Badizadegan, Kamran; Dasari, Ramachandra R.; Feld, Michael S.

In: Journal of Physical Chemistry A, Vol. 113, No. 47, 01.12.2009, p. 13327-13330.

Research output: Contribution to journalArticle

Lue, N, Choi, W, Popescu, G, Yaqoob, Z, Badizadegan, K, Dasari, RR & Feld, MS 2009, 'Live cell refractometry using hilbert phase microscopy and confocal reflectance microscopy', Journal of Physical Chemistry A, vol. 113, no. 47, pp. 13327-13330. https://doi.org/10.1021/jp904746r
Lue, Niyom ; Choi, Wonshik ; Popescu, Gabriel ; Yaqoob, Zahid ; Badizadegan, Kamran ; Dasari, Ramachandra R. ; Feld, Michael S. / Live cell refractometry using hilbert phase microscopy and confocal reflectance microscopy. In: Journal of Physical Chemistry A. 2009 ; Vol. 113, No. 47. pp. 13327-13330.
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