Localization of PDGF-stimulated phosphorylation cascade to caveolae

Pinesheng Liu, Yunshu Vine, Young-Gyu Ko, R. G W Andersen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Previously we showed that IL-Iβ stimulates the conversion of sphingomyelin to ceramide in the caveolae fraction of normal human fibroblasts. The ceramide, in turn, blocked PDGF-stimulated DNA synthesis. We now present evidence that the PDGF receptor initiates signal transduction from caveolae. Cell fractionation and immunocytochemistry show caveolae to be the principal location of PDGF receptors at the cell surface. Multiple caveolae proteins acquire phosphotyrosine when PDGF binds to its receptor, but the hormone appears to have little effect on the tyrosine phosphorylation of noncaveolae membrane proteins. Five proteins known to interact with the phosphorylated receptor were found to be highly enriched in caveolae membrane. PDGF caused the concentration of three of these proteins to significantly increase in the caveolae fraction. Finally, PDGF stimulated the association of a 190 kDa phosphoprotein with the caveolae marker protein, caveolin. Therefore, ceramide may modulate PDGF receptor function directly in caveolae.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number6
Publication statusPublished - 1996 Dec 1
Externally publishedYes

Fingerprint

Platelet-Derived Growth Factor Receptors
Caveolae
Phosphorylation
Ceramides
ceramides
phosphorylation
Caveolins
Signal transduction
Phosphotyrosine
Proteins
Sphingomyelins
proteins
Phosphoproteins
cell fractionation
Fibroblasts
Fractionation
sphingomyelins
phosphoproteins
Tyrosine
hormone receptors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Localization of PDGF-stimulated phosphorylation cascade to caveolae. / Liu, Pinesheng; Vine, Yunshu; Ko, Young-Gyu; Andersen, R. G W.

In: FASEB Journal, Vol. 10, No. 6, 01.12.1996.

Research output: Contribution to journalArticle

Liu, Pinesheng ; Vine, Yunshu ; Ko, Young-Gyu ; Andersen, R. G W. / Localization of PDGF-stimulated phosphorylation cascade to caveolae. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
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