Localization of platelet-derived growth factor-stimulated phosphorylation cascade to caveolae

Pingsheng Liu, Yunshu Ying, Young Gyu Ko, Richard G.W. Anderson

Research output: Contribution to journalArticle

330 Citations (Scopus)

Abstract

Previously we showed that interleukin 1β stimulates the conversion of sphingomyelin to ceramide in the caveolae fraction of normal human fibroblasts. The ceramide, in turn, blocked platelet-derived growth factor (PDGF) stimulated DNA synthesis. We now present evidence that the PDGF receptor initiates signal transduction from caveolae. Cell fractionation and immunocytochemistry show caveolae to be the principal location of PDGF receptors at the cell surface. Multiple caveolae proteins acquire phosphotyrosine when PDGF binds to its receptor, but the hormone appears to have little effect on the tyrosine phosphorylation of non-caveolae membrane proteins. Five proteins known to interact with the phosphorylated receptor were found to be highly enriched in caveolae membrane. PDGF caused the concentration of three of these proteins to significantly increase in the caveolae fraction. Finally, PDGF stimulated the association of a 190-kDa phosphoprotein with the caveolae marker protein, caveolin. Therefore, ceramide may modulate PDGF receptor function directly in caveolae.

Original languageEnglish
Pages (from-to)10299-10303
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number17
DOIs
Publication statusPublished - 1996 Apr 26

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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