Localization of two forms of phospholipase C-β1, a and b, in C6Bu-1 cells

Young Yil Bahk, Hebok Song, Suk Hwan Baek, Byung Yu Park, Hyun Kim, Sung Ho Ryu, Pann Ghill Suh

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Phospholipase C-β1 (PLC-β1), one of the PLC-β isozymes, exists as two immunologically distinguishable polypeptides of 150 (PLC-β1a) and 140 kDa (PLC-β1b) which are encoded in two distinct transcripts and generated by alternative splicing of a single gene. In this study, the subcellular localization of the two phospholipases C-β1 proteins was examined in rat C6Bu-1 glioma cells using immunological techniques. Immunoblot analysis revealed that the two forms of PLC-β1 were detectable in both cytosolic and nuclear fractions. PLC-β1a appeared to be located preferentially in the cytosol, whereas PLC-β1b was found predominantly in the nuclei of C6Bu-1 cells. Immunocytochemical experiments confirmed the differential localization of the: two PLC-β1 species in C6Bu-1 cells. These results suggest that the two PLC-β1 proteins may have different physiological roles in the cell.

Original languageEnglish
Pages (from-to)76-80
Number of pages5
JournalBiochimica et Biophysica Acta - Lipids and Lipid Metabolism
Volume1389
Issue number1
DOIs
Publication statusPublished - 1998 Jan 5

    Fingerprint

Keywords

  • C6Bu-1 cell
  • Immunocytochemistry
  • Nuclear localization
  • Phospholipase C-β1

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Biophysics

Cite this