Localized electroporation effect on adherent cells in modified electric cell-substrate impedance sensing circuits

Yu Jin Kim, Ka Ram Song, Hee Dae Kim, Bum Chul Park, Young-geun Kim, Chi Jung Kang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Electroporation is a physical transfection method for introducing foreign genes or drugs into cells. It does not require toxic reagents or transfection vectors. However, its applications have been limited because of cell damage and nonspecific transport. Here, we present an effective method for selective and localized electroporation using atomic force microscopy. This electroporation method is applied to adherent cells on substrates, instead of conventionally used suspended cells, and offers relatively effective cell transfection. Moreover, this method enables localized transfection into targeted areas at the single-cell level.

Original languageEnglish
Article number107001
JournalApplied Physics Express
Volume9
Issue number10
DOIs
Publication statusPublished - 2016 Oct 1

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electric cells
Electric batteries
Atomic force microscopy
Genes
Cells
impedance
Networks (circuits)
Substrates
cells
genes
reagents
drugs
atomic force microscopy
damage

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Localized electroporation effect on adherent cells in modified electric cell-substrate impedance sensing circuits. / Kim, Yu Jin; Song, Ka Ram; Kim, Hee Dae; Park, Bum Chul; Kim, Young-geun; Kang, Chi Jung.

In: Applied Physics Express, Vol. 9, No. 10, 107001, 01.10.2016.

Research output: Contribution to journalArticle

Kim, Yu Jin ; Song, Ka Ram ; Kim, Hee Dae ; Park, Bum Chul ; Kim, Young-geun ; Kang, Chi Jung. / Localized electroporation effect on adherent cells in modified electric cell-substrate impedance sensing circuits. In: Applied Physics Express. 2016 ; Vol. 9, No. 10.
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