TY - JOUR
T1 - Localized surface plasmon resonance aptasensor for the highly sensitive direct detection of cortisol in human saliva
AU - Jo, Seongjae
AU - Lee, Wonseok
AU - Park, Joohyung
AU - Kim, Woong
AU - Kim, Woochang
AU - Lee, Gyudo
AU - Lee, Heon Jeong
AU - Hong, Junghwa
AU - Park, Jinsung
N1 - Funding Information:
This work was supported by a National Research Foundation of Korea (NRF) grant , funded by the Korean government ( NRF-2017M3A9F1031229 , NRF-2017R1E1A1A01075439 and NRF-2019R1A2C3009595 ) and was conducted with the support of the Korea Environment Industry & Technology Institute (KEITI) through its Ecological Imitation-based Environmental Pollution Management Technology Development Project, and funded by the Korea Ministry of Environment (MOE) ( 2019002800009 ) Appendix A
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Cortisol, which is a stress biomarker that causes various mental disorders, is found in sweat, saliva, blood, and urine. Because mental disorders resulting from stress are a silent contributor to various diseases, the detection of cortisol level is an important step in their prevention. Here, we developed a highly sensitive, localized surface plasmon resonance (LSPR) aptasensor for the direct detection of cortisol in saliva. The LSPR aptasensor was constructed using gold nanoparticles of different sizes. Subsequently, the surface of the LSPR substrate was immobilized using an aptamer for capturing cortisol. The detection capability of the LSPR aptasensor was compared with that of the antibody- immobilized sensor. The LSPR aptasensor displayed excellent detection capability for a wide range of cortisol concentrations extending from 0.1–1000 nM with a detection limit of 0.1 nM. In addition, the LSPR aptasensor was able to selectively detect cortisol among other substances with similar chemical structures (e.g., cortisone, corticosterone, progesterone, and triamcinolone). These results indicate that the LSPR aptasensor may be used for the highly sensitive and selective detection of cortisol in salivary samples.
AB - Cortisol, which is a stress biomarker that causes various mental disorders, is found in sweat, saliva, blood, and urine. Because mental disorders resulting from stress are a silent contributor to various diseases, the detection of cortisol level is an important step in their prevention. Here, we developed a highly sensitive, localized surface plasmon resonance (LSPR) aptasensor for the direct detection of cortisol in saliva. The LSPR aptasensor was constructed using gold nanoparticles of different sizes. Subsequently, the surface of the LSPR substrate was immobilized using an aptamer for capturing cortisol. The detection capability of the LSPR aptasensor was compared with that of the antibody- immobilized sensor. The LSPR aptasensor displayed excellent detection capability for a wide range of cortisol concentrations extending from 0.1–1000 nM with a detection limit of 0.1 nM. In addition, the LSPR aptasensor was able to selectively detect cortisol among other substances with similar chemical structures (e.g., cortisone, corticosterone, progesterone, and triamcinolone). These results indicate that the LSPR aptasensor may be used for the highly sensitive and selective detection of cortisol in salivary samples.
KW - Aptasensor
KW - Cortisol
KW - Gold nanoparticles
KW - Human saliva
KW - Localized surface plasmon resonance
UR - http://www.scopus.com/inward/record.url?scp=85075776937&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2019.127424
DO - 10.1016/j.snb.2019.127424
M3 - Article
AN - SCOPUS:85075776937
VL - 304
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
M1 - 127424
ER -