Long-term local PDGF delivery using porous microspheres modified with heparin for tendon healing of rotator cuff tendinitis in a rabbit model

Seok Kang, Joon Shik Yoon, Jae Yong Lee, Hak Jun Kim, Kyeongsoon Park, Sung Eun Kim

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    In this study, we prepared the platelet-derived growth factor-containing porous microspheres modified with heparin (PDGF/Hep-PMSs) and investigated their anti-inflammatory and tendon healing effects on rotator cuff (RC) tendinitis rabbit model. PDGF/Hep-PMSs suppressed the mRNA levels of six pro-inflammatory cytokines (i.e., MMP-3, MMP-13, COX-2, ADAMTS-5, IL-6, and TNF-α) in inflamed tenocytes. Long-term local delivery of PDGF/Hep-PMSs into tendon tissues of RC tendinitis decreased the mRNA levels of six pro-inflammatory cytokines and increased the mRNA levels of anti-inflammatory cytokines including IL-4, IL-10, and IL-13. Anti-inflammatory effects of PDGF/Hep-PMSs might have contributed to enhance the collagen content, tenogenic markers, stiffness, and tensile strength of tendons, eventually leading to tendon restoration. Our findings suggest that the long-term local PDGF delivery of PDGF/Hep-PMSs have a great potential to enhance tendon healing of RC tendinitis by suppressing inflammation responses.

    Original languageEnglish
    Pages (from-to)372-381
    Number of pages10
    JournalCarbohydrate Polymers
    Volume209
    DOIs
    Publication statusPublished - 2019 Apr 1

    Keywords

    • 2-(N-morpholino)-ethanesulfonic acid (Pubchem CID:4478249)
    • Anti-inflammation
    • Dichloromethane (DCM) (PubChem CID: 6344)
    • Dimethylsulfoxide (DMSO) (PubChem CID: 679)
    • Dopamine hydrochloride (PubChem CID:65340)
    • Heparin
    • Heparin sodium (PubChem CID:22833565)
    • PDGF
    • Poly (lactic-co-glycolic acid) (PLGA) (Pubchem CID: 23111554)
    • Polyvinyl alcohol (PVA) (PubChem CID: 11199)
    • Porous microspheres
    • Recombinant human PDGF (PubChem SID: 135290697)
    • Rotator cuff tendinitis
    • Sodium chloride (PubChem CID:5234)
    • Tendon healing
    • Thiazolyl blue tetrazolium bromide (MTT) (PubChem CID:64965)

    ASJC Scopus subject areas

    • Organic Chemistry
    • Polymers and Plastics
    • Materials Chemistry

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