Long-term local PDGF delivery using porous microspheres modified with heparin for tendon healing of rotator cuff tendinitis in a rabbit model

Seok Kang, Joon Shik Yoon, Jae Yong Lee, Hak Jun Kim, Kyeongsoon Park, Sung Eun Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In this study, we prepared the platelet-derived growth factor-containing porous microspheres modified with heparin (PDGF/Hep-PMSs) and investigated their anti-inflammatory and tendon healing effects on rotator cuff (RC) tendinitis rabbit model. PDGF/Hep-PMSs suppressed the mRNA levels of six pro-inflammatory cytokines (i.e., MMP-3, MMP-13, COX-2, ADAMTS-5, IL-6, and TNF-α) in inflamed tenocytes. Long-term local delivery of PDGF/Hep-PMSs into tendon tissues of RC tendinitis decreased the mRNA levels of six pro-inflammatory cytokines and increased the mRNA levels of anti-inflammatory cytokines including IL-4, IL-10, and IL-13. Anti-inflammatory effects of PDGF/Hep-PMSs might have contributed to enhance the collagen content, tenogenic markers, stiffness, and tensile strength of tendons, eventually leading to tendon restoration. Our findings suggest that the long-term local PDGF delivery of PDGF/Hep-PMSs have a great potential to enhance tendon healing of RC tendinitis by suppressing inflammation responses.

Original languageEnglish
Pages (from-to)372-381
Number of pages10
JournalCarbohydrate Polymers
Volume209
DOIs
Publication statusPublished - 2019 Apr 1

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Keywords

  • 2-(N-morpholino)-ethanesulfonic acid (Pubchem CID:4478249)
  • Anti-inflammation
  • Dichloromethane (DCM) (PubChem CID: 6344)
  • Dimethylsulfoxide (DMSO) (PubChem CID: 679)
  • Dopamine hydrochloride (PubChem CID:65340)
  • Heparin
  • Heparin sodium (PubChem CID:22833565)
  • PDGF
  • Poly (lactic-co-glycolic acid) (PLGA) (Pubchem CID: 23111554)
  • Polyvinyl alcohol (PVA) (PubChem CID: 11199)
  • Porous microspheres
  • Recombinant human PDGF (PubChem SID: 135290697)
  • Rotator cuff tendinitis
  • Sodium chloride (PubChem CID:5234)
  • Tendon healing
  • Thiazolyl blue tetrazolium bromide (MTT) (PubChem CID:64965)

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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