Long term storage of dry versus frozen RNA for next generation molecular studies

Eric Seelenfreund, William A. Robinson, Carol M. Amato, Aik-Choon Tan, Jihye Kim, Steven E. Robinson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The standard method for the storage and preservation of RNA has been at ultra-low temperatures. However, reliance on liquid nitrogen and freezers for storage of RNA has multiple downsides. Recently new techniques have been developed for storing RNA at room temperature utilizing desiccation and are reported to be an effective alternative for preserving RNA integrity. In this study we compared frozen RNA samples stored for up to one year to those which had been desiccated using RNAstable (Biomatrica, Inc., San Diego, CA) and stored at room temperature. RNA samples were placed in aliquots and stored after desiccation or frozen (at 280°C), and were analyzed for RNA Integrity Number (RIN), and by qPCR, and RNA sequencing. Our study shows that RNAstable is able to preserve desiccated RNA samples at room temperature for up to one year, and that RNA preserved by desiccation is comparable to cryopreserved RNA for downstream analyses including realtime-PCR and RNA sequencing.

Original languageEnglish
Article numbere111827
JournalPLoS One
Volume9
Issue number11
DOIs
Publication statusPublished - 2014 Nov 7
Externally publishedYes

Fingerprint

RNA
Desiccation
RNA Sequence Analysis
ambient temperature
Temperature
sequence analysis
freezers
sampling
Liquid nitrogen
preserves
Nitrogen
Polymerase Chain Reaction
liquids
nitrogen
temperature

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Seelenfreund, E., Robinson, W. A., Amato, C. M., Tan, A-C., Kim, J., & Robinson, S. E. (2014). Long term storage of dry versus frozen RNA for next generation molecular studies. PLoS One, 9(11), [e111827]. https://doi.org/10.1371/journal.pone.0111827

Long term storage of dry versus frozen RNA for next generation molecular studies. / Seelenfreund, Eric; Robinson, William A.; Amato, Carol M.; Tan, Aik-Choon; Kim, Jihye; Robinson, Steven E.

In: PLoS One, Vol. 9, No. 11, e111827, 07.11.2014.

Research output: Contribution to journalArticle

Seelenfreund, E, Robinson, WA, Amato, CM, Tan, A-C, Kim, J & Robinson, SE 2014, 'Long term storage of dry versus frozen RNA for next generation molecular studies', PLoS One, vol. 9, no. 11, e111827. https://doi.org/10.1371/journal.pone.0111827
Seelenfreund E, Robinson WA, Amato CM, Tan A-C, Kim J, Robinson SE. Long term storage of dry versus frozen RNA for next generation molecular studies. PLoS One. 2014 Nov 7;9(11). e111827. https://doi.org/10.1371/journal.pone.0111827
Seelenfreund, Eric ; Robinson, William A. ; Amato, Carol M. ; Tan, Aik-Choon ; Kim, Jihye ; Robinson, Steven E. / Long term storage of dry versus frozen RNA for next generation molecular studies. In: PLoS One. 2014 ; Vol. 9, No. 11.
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