Longitudinal monitoring adipose-derived stem cell survival by PET imaging hexadecyl-4-124I-iodobenzoate in rat myocardial infarction model

Min Hwan Kim, Sang Keun Woo, Kyo Chul Lee, Gwang Il An, Darpan Pandya, Noh Won Park, Sang Soep Nahm, Ki Dong Eom, Kwang Il Kim, Tae Sup Lee, Chan Wha Kim, Joo Hyun Kang, Jeongsoo Yoo, Yong Jin Lee

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

This study aims to monitor how the change of cell survival of transplanted adipose-derived stem cells (ADSCs) responds to myocardial infarction (MI) via the hexadecyl-4-124I-iodobenzoate (124I-HIB) mediated direct labeling method in vivo. Stem cells have shown the potential to improve cardiac function after MI. However, monitoring of the fate of transplanted stem cells at target sites is still unclear. Rat ADSCs were labeled with 124I-HIB, and radiolabeled ADSCs were transplanted into the myocardium of normal and MI model. In the group of 124I-HIB-labeled ADSC transplantation, in vivo imaging was performed using small-animal positron emission tomography (PET)/computed tomography (CT) for 9 days. Twenty-one days post-transplantation, histopathological analysis and apoptosis assay were performed. ADSC viability and differentiation were not affected by 124I-HIB labeling. In vivo tracking of the 124I-HIB-labeled ADSCs was possible for 9 and 3 days in normal and MI model, respectively. Apoptosis of transplanted cells increased in the MI model compared than that in normal model. We developed a direct labeling agent, 124I-HIB, and first tried to longitudinally monitor transplanted stem cell to MI. This approach may provide new insights on the roles of stem cell monitoring in living bodies for stem cell therapy from pre-clinical studies to clinical trials.

Original languageEnglish
Pages (from-to)13-19
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume456
Issue number1
DOIs
Publication statusPublished - 2015 Jan 2

Keywords

  • Adipose-derived stem cells
  • Cell tracking
  • Direct labeling agent
  • Hexadecyl-4-I-iodobenzoate
  • Myocardial infarction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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