LOS2, a genetic locus required for cold-responsive gene transcription encodes a bi-functional enolase

Hojoung Lee, Yan Guo, Masaru Ohta, Liming Xiong, Becky Stevenson, Jian Kang Zhu

Research output: Contribution to journalArticle

234 Citations (Scopus)

Abstract

The Arabidopsis mutation, los2, impairs cold-responsive gene transcription, acquired freezing tolerance and plant resistance to chilling under certain conditions. LOS2 was isolated through positional cloning and shown to encode an enolase in the glycolytic pathway. In animal cells, enolase has also been known to function as a transcription factor that represses the expression of c-myc by binding to the c-myc gene promoter. LOS2 fused to green fluorescent protein is targeted to the nucleus as well as to the cytoplasm. LOS2/enolase protein can bind to the cis-element of the human c-myc gene promoter and to the gene promoter of STZ/ZAT10, a zinc finger transcriptional repressor from Arabidopsis. STZ/ZAT10 expression is induced rapidly and transiently by cold in the wild type, and this induction is stronger and more sustained in the los2 mutant. Furthermore, the expression of a RD29A-LUC reporter gene is repressed significantly by STZ/ZAT10 in transient expression assays in Arabidopsis leaves. Our results demonstrate that cold-responsive gene transcription in plants is controlled by a bi-functional enolase.

Original languageEnglish
Pages (from-to)2692-2702
Number of pages11
JournalEMBO Journal
Volume21
Issue number11
DOIs
Publication statusPublished - 2002 Jun 3
Externally publishedYes

Fingerprint

Genetic Loci
Phosphopyruvate Hydratase
Transcription
Genes
Arabidopsis
myc Genes
Zinc Fingers
Green Fluorescent Proteins
Reporter Genes
Freezing
Chilling
Organism Cloning
Cytoplasm
Transcription Factors
Cloning
Mutation
Zinc
Assays
Animals
Cells

Keywords

  • Cold signaling
  • Enolase
  • LOS2
  • Transcription factor
  • ZAT10 (STZ)

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

LOS2, a genetic locus required for cold-responsive gene transcription encodes a bi-functional enolase. / Lee, Hojoung; Guo, Yan; Ohta, Masaru; Xiong, Liming; Stevenson, Becky; Zhu, Jian Kang.

In: EMBO Journal, Vol. 21, No. 11, 03.06.2002, p. 2692-2702.

Research output: Contribution to journalArticle

Lee, Hojoung ; Guo, Yan ; Ohta, Masaru ; Xiong, Liming ; Stevenson, Becky ; Zhu, Jian Kang. / LOS2, a genetic locus required for cold-responsive gene transcription encodes a bi-functional enolase. In: EMBO Journal. 2002 ; Vol. 21, No. 11. pp. 2692-2702.
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