Loss of CDC14B expression in clear cell renal cell carcinoma: Meta-analysis of microarray data sets

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4 Citations (Scopus)

Abstract

Objectives: To discover significant differentially expressed genes (DEGs) in clear cell renal cell carcinoma (ccRCC) that might be unidentified by single microarray analysis. Methods: The effect sizes of five ccRCC microarray data sets were combined using a random-effects model. The most downregulated gene was validated in paired 80 ccRCC tissues by immunohistochemistry. Results: CDC14B was the most downregulated gene among 1,761 DEGs. CDC14B was strongly expressed in the apical proximal tubules in the nonneoplastic tissues, while it was completely absent in 10 (12.5%) of 80 or downregulated in 70 (87.5%) of 80 ccRCC cases. The complete loss of CDC14B correlated with high T stage (P = .038), advanced TNM stage (P = .027), tumor recurrence (P = .038), and shorter recurrence-free survival (P = .046) compared with the partial loss of CDC14B. Conclusions: Microarray meta-analysis is a useful tool for pathologists. CDC14B expression is downregulated in ccRCC, suggesting its role in renal carcinogenesis.

Original languageEnglish
Pages (from-to)551-558
Number of pages8
JournalAmerican Journal of Clinical Pathology
Volume141
Issue number4
DOIs
Publication statusPublished - 2014 Jan 1

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Renal Cell Carcinoma
Meta-Analysis
Down-Regulation
Microarray Analysis
Genes
Recurrence
Carcinogenesis
Immunohistochemistry
Datasets
Kidney
Neoplasms

Keywords

  • CDC14B
  • Meta-analysis
  • Microarray
  • Renal cell carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

@article{20a5e1831b014657b475e01ac548e699,
title = "Loss of CDC14B expression in clear cell renal cell carcinoma: Meta-analysis of microarray data sets",
abstract = "Objectives: To discover significant differentially expressed genes (DEGs) in clear cell renal cell carcinoma (ccRCC) that might be unidentified by single microarray analysis. Methods: The effect sizes of five ccRCC microarray data sets were combined using a random-effects model. The most downregulated gene was validated in paired 80 ccRCC tissues by immunohistochemistry. Results: CDC14B was the most downregulated gene among 1,761 DEGs. CDC14B was strongly expressed in the apical proximal tubules in the nonneoplastic tissues, while it was completely absent in 10 (12.5{\%}) of 80 or downregulated in 70 (87.5{\%}) of 80 ccRCC cases. The complete loss of CDC14B correlated with high T stage (P = .038), advanced TNM stage (P = .027), tumor recurrence (P = .038), and shorter recurrence-free survival (P = .046) compared with the partial loss of CDC14B. Conclusions: Microarray meta-analysis is a useful tool for pathologists. CDC14B expression is downregulated in ccRCC, suggesting its role in renal carcinogenesis.",
keywords = "CDC14B, Meta-analysis, Microarray, Renal cell carcinoma",
author = "Younghye Kim and Jung-Woo Choi and Ju-Han Lee and Kim, {Young Sik}",
year = "2014",
month = "1",
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doi = "10.1309/AJCP4PE4JPSRGBQS",
language = "English",
volume = "141",
pages = "551--558",
journal = "American Journal of Clinical Pathology",
issn = "0002-9173",
publisher = "American Society of Clinical Pathologists",
number = "4",

}

TY - JOUR

T1 - Loss of CDC14B expression in clear cell renal cell carcinoma

T2 - Meta-analysis of microarray data sets

AU - Kim, Younghye

AU - Choi, Jung-Woo

AU - Lee, Ju-Han

AU - Kim, Young Sik

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Objectives: To discover significant differentially expressed genes (DEGs) in clear cell renal cell carcinoma (ccRCC) that might be unidentified by single microarray analysis. Methods: The effect sizes of five ccRCC microarray data sets were combined using a random-effects model. The most downregulated gene was validated in paired 80 ccRCC tissues by immunohistochemistry. Results: CDC14B was the most downregulated gene among 1,761 DEGs. CDC14B was strongly expressed in the apical proximal tubules in the nonneoplastic tissues, while it was completely absent in 10 (12.5%) of 80 or downregulated in 70 (87.5%) of 80 ccRCC cases. The complete loss of CDC14B correlated with high T stage (P = .038), advanced TNM stage (P = .027), tumor recurrence (P = .038), and shorter recurrence-free survival (P = .046) compared with the partial loss of CDC14B. Conclusions: Microarray meta-analysis is a useful tool for pathologists. CDC14B expression is downregulated in ccRCC, suggesting its role in renal carcinogenesis.

AB - Objectives: To discover significant differentially expressed genes (DEGs) in clear cell renal cell carcinoma (ccRCC) that might be unidentified by single microarray analysis. Methods: The effect sizes of five ccRCC microarray data sets were combined using a random-effects model. The most downregulated gene was validated in paired 80 ccRCC tissues by immunohistochemistry. Results: CDC14B was the most downregulated gene among 1,761 DEGs. CDC14B was strongly expressed in the apical proximal tubules in the nonneoplastic tissues, while it was completely absent in 10 (12.5%) of 80 or downregulated in 70 (87.5%) of 80 ccRCC cases. The complete loss of CDC14B correlated with high T stage (P = .038), advanced TNM stage (P = .027), tumor recurrence (P = .038), and shorter recurrence-free survival (P = .046) compared with the partial loss of CDC14B. Conclusions: Microarray meta-analysis is a useful tool for pathologists. CDC14B expression is downregulated in ccRCC, suggesting its role in renal carcinogenesis.

KW - CDC14B

KW - Meta-analysis

KW - Microarray

KW - Renal cell carcinoma

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