Abstract
Objectives: To discover significant differentially expressed genes (DEGs) in clear cell renal cell carcinoma (ccRCC) that might be unidentified by single microarray analysis. Methods: The effect sizes of five ccRCC microarray data sets were combined using a random-effects model. The most downregulated gene was validated in paired 80 ccRCC tissues by immunohistochemistry. Results: CDC14B was the most downregulated gene among 1,761 DEGs. CDC14B was strongly expressed in the apical proximal tubules in the nonneoplastic tissues, while it was completely absent in 10 (12.5%) of 80 or downregulated in 70 (87.5%) of 80 ccRCC cases. The complete loss of CDC14B correlated with high T stage (P = .038), advanced TNM stage (P = .027), tumor recurrence (P = .038), and shorter recurrence-free survival (P = .046) compared with the partial loss of CDC14B. Conclusions: Microarray meta-analysis is a useful tool for pathologists. CDC14B expression is downregulated in ccRCC, suggesting its role in renal carcinogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 551-558 |
| Number of pages | 8 |
| Journal | American Journal of Clinical Pathology |
| Volume | 141 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2014 Jan 1 |
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Keywords
- CDC14B
- Meta-analysis
- Microarray
- Renal cell carcinoma
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Loss of CDC14B expression in clear cell renal cell carcinoma : Meta-analysis of microarray data sets. / Kim, Younghye; Choi, Jung-Woo; Lee, Ju-Han; Kim, Young Sik.
In: American Journal of Clinical Pathology, Vol. 141, No. 4, 01.01.2014, p. 551-558.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Loss of CDC14B expression in clear cell renal cell carcinoma
T2 - Meta-analysis of microarray data sets
AU - Kim, Younghye
AU - Choi, Jung-Woo
AU - Lee, Ju-Han
AU - Kim, Young Sik
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Objectives: To discover significant differentially expressed genes (DEGs) in clear cell renal cell carcinoma (ccRCC) that might be unidentified by single microarray analysis. Methods: The effect sizes of five ccRCC microarray data sets were combined using a random-effects model. The most downregulated gene was validated in paired 80 ccRCC tissues by immunohistochemistry. Results: CDC14B was the most downregulated gene among 1,761 DEGs. CDC14B was strongly expressed in the apical proximal tubules in the nonneoplastic tissues, while it was completely absent in 10 (12.5%) of 80 or downregulated in 70 (87.5%) of 80 ccRCC cases. The complete loss of CDC14B correlated with high T stage (P = .038), advanced TNM stage (P = .027), tumor recurrence (P = .038), and shorter recurrence-free survival (P = .046) compared with the partial loss of CDC14B. Conclusions: Microarray meta-analysis is a useful tool for pathologists. CDC14B expression is downregulated in ccRCC, suggesting its role in renal carcinogenesis.
AB - Objectives: To discover significant differentially expressed genes (DEGs) in clear cell renal cell carcinoma (ccRCC) that might be unidentified by single microarray analysis. Methods: The effect sizes of five ccRCC microarray data sets were combined using a random-effects model. The most downregulated gene was validated in paired 80 ccRCC tissues by immunohistochemistry. Results: CDC14B was the most downregulated gene among 1,761 DEGs. CDC14B was strongly expressed in the apical proximal tubules in the nonneoplastic tissues, while it was completely absent in 10 (12.5%) of 80 or downregulated in 70 (87.5%) of 80 ccRCC cases. The complete loss of CDC14B correlated with high T stage (P = .038), advanced TNM stage (P = .027), tumor recurrence (P = .038), and shorter recurrence-free survival (P = .046) compared with the partial loss of CDC14B. Conclusions: Microarray meta-analysis is a useful tool for pathologists. CDC14B expression is downregulated in ccRCC, suggesting its role in renal carcinogenesis.
KW - CDC14B
KW - Meta-analysis
KW - Microarray
KW - Renal cell carcinoma
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UR - http://www.scopus.com/inward/citedby.url?scp=84899471393&partnerID=8YFLogxK
U2 - 10.1309/AJCP4PE4JPSRGBQS
DO - 10.1309/AJCP4PE4JPSRGBQS
M3 - Article
C2 - 24619757
AN - SCOPUS:84899471393
VL - 141
SP - 551
EP - 558
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
SN - 0002-9173
IS - 4
ER -