Abstract
The protein product of Saccharomyces cerevisiaeDFG5 gene is a glycosylphosphatidylinositol (GPI)-anchored plasma membrane protein and a putative glycosidase/glycosyltransferase that links other GPI-anchored proteins to β-glucans in the cell wall. Upon exposure to heat (41°C), DFG5 deletion mutant dfg5Δ displayed significantly enhanced heat tolerance as well as lowered level of reactive oxygen species and decreased membrane permeability compared with those in the control (BY4741). Comparative transcriptome profiles of BY4741 and dfg5Δ revealed that 38 and 23 genes were up- and down-regulated in dfg5Δ respectively. Of the 23 down-regulated genes, 11 of 13 viable deletion mutants were identified to be tolerant to heat, suggesting that the down-regulation of those genes might have contributed to the enhanced heat tolerance in dfg5Δ. Deletion of DFG5 caused slight activation of mitogen-activated protein kinases Hog1 in the high-osmolarity glycerol pathway and Slt2 in the cell wall integrity pathway. Therefore, a model is proposed on the signal transduction pathways associated with deletion of DFG5 upon heat stress.
| Original language | English |
|---|---|
| Pages (from-to) | 2721-2734 |
| Number of pages | 14 |
| Journal | Environmental Microbiology |
| Volume | 17 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2015 Aug 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology
- Ecology, Evolution, Behavior and Systematics
Cite this
Loss of Dfg5 glycosylphosphatidylinositol-anchored membrane protein confers enhanced heat tolerance in Saccharomyces cerevisiae. / Nasution, Olviyani; Lee, Jaok; Srinivasa, Kavitha; Choi, In-Geol; Lee, Young Mi; Kim, Eunjung; Choi, Wonja; Kim, Wankee.
In: Environmental Microbiology, Vol. 17, No. 8, 01.08.2015, p. 2721-2734.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Loss of Dfg5 glycosylphosphatidylinositol-anchored membrane protein confers enhanced heat tolerance in Saccharomyces cerevisiae
AU - Nasution, Olviyani
AU - Lee, Jaok
AU - Srinivasa, Kavitha
AU - Choi, In-Geol
AU - Lee, Young Mi
AU - Kim, Eunjung
AU - Choi, Wonja
AU - Kim, Wankee
PY - 2015/8/1
Y1 - 2015/8/1
N2 - The protein product of Saccharomyces cerevisiaeDFG5 gene is a glycosylphosphatidylinositol (GPI)-anchored plasma membrane protein and a putative glycosidase/glycosyltransferase that links other GPI-anchored proteins to β-glucans in the cell wall. Upon exposure to heat (41°C), DFG5 deletion mutant dfg5Δ displayed significantly enhanced heat tolerance as well as lowered level of reactive oxygen species and decreased membrane permeability compared with those in the control (BY4741). Comparative transcriptome profiles of BY4741 and dfg5Δ revealed that 38 and 23 genes were up- and down-regulated in dfg5Δ respectively. Of the 23 down-regulated genes, 11 of 13 viable deletion mutants were identified to be tolerant to heat, suggesting that the down-regulation of those genes might have contributed to the enhanced heat tolerance in dfg5Δ. Deletion of DFG5 caused slight activation of mitogen-activated protein kinases Hog1 in the high-osmolarity glycerol pathway and Slt2 in the cell wall integrity pathway. Therefore, a model is proposed on the signal transduction pathways associated with deletion of DFG5 upon heat stress.
AB - The protein product of Saccharomyces cerevisiaeDFG5 gene is a glycosylphosphatidylinositol (GPI)-anchored plasma membrane protein and a putative glycosidase/glycosyltransferase that links other GPI-anchored proteins to β-glucans in the cell wall. Upon exposure to heat (41°C), DFG5 deletion mutant dfg5Δ displayed significantly enhanced heat tolerance as well as lowered level of reactive oxygen species and decreased membrane permeability compared with those in the control (BY4741). Comparative transcriptome profiles of BY4741 and dfg5Δ revealed that 38 and 23 genes were up- and down-regulated in dfg5Δ respectively. Of the 23 down-regulated genes, 11 of 13 viable deletion mutants were identified to be tolerant to heat, suggesting that the down-regulation of those genes might have contributed to the enhanced heat tolerance in dfg5Δ. Deletion of DFG5 caused slight activation of mitogen-activated protein kinases Hog1 in the high-osmolarity glycerol pathway and Slt2 in the cell wall integrity pathway. Therefore, a model is proposed on the signal transduction pathways associated with deletion of DFG5 upon heat stress.
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U2 - 10.1111/1462-2920.12649
DO - 10.1111/1462-2920.12649
M3 - Article
C2 - 25297926
AN - SCOPUS:84939266035
VL - 17
SP - 2721
EP - 2734
JO - Environmental Microbiology
JF - Environmental Microbiology
SN - 1462-2912
IS - 8
ER -