Loss of Dfg5 glycosylphosphatidylinositol-anchored membrane protein confers enhanced heat tolerance in Saccharomyces cerevisiae

Olviyani Nasution, Jaok Lee, Kavitha Srinivasa, In-Geol Choi, Young Mi Lee, Eunjung Kim, Wonja Choi, Wankee Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The protein product of Saccharomyces cerevisiaeDFG5 gene is a glycosylphosphatidylinositol (GPI)-anchored plasma membrane protein and a putative glycosidase/glycosyltransferase that links other GPI-anchored proteins to β-glucans in the cell wall. Upon exposure to heat (41°C), DFG5 deletion mutant dfg5Δ displayed significantly enhanced heat tolerance as well as lowered level of reactive oxygen species and decreased membrane permeability compared with those in the control (BY4741). Comparative transcriptome profiles of BY4741 and dfg5Δ revealed that 38 and 23 genes were up- and down-regulated in dfg5Δ respectively. Of the 23 down-regulated genes, 11 of 13 viable deletion mutants were identified to be tolerant to heat, suggesting that the down-regulation of those genes might have contributed to the enhanced heat tolerance in dfg5Δ. Deletion of DFG5 caused slight activation of mitogen-activated protein kinases Hog1 in the high-osmolarity glycerol pathway and Slt2 in the cell wall integrity pathway. Therefore, a model is proposed on the signal transduction pathways associated with deletion of DFG5 upon heat stress.

Original languageEnglish
Pages (from-to)2721-2734
Number of pages14
JournalEnvironmental Microbiology
Volume17
Issue number8
DOIs
Publication statusPublished - 2015 Aug 1
Externally publishedYes

Fingerprint

Glycosylphosphatidylinositols
heat tolerance
membrane proteins
Saccharomyces cerevisiae
Membrane Proteins
tolerance
membrane
protein
gene
Hot Temperature
Cell Wall
Genes
genes
cell walls
heat
Glycosyltransferases
mutants
glycosyltransferases
Saccharomyces
osmolarity

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Loss of Dfg5 glycosylphosphatidylinositol-anchored membrane protein confers enhanced heat tolerance in Saccharomyces cerevisiae. / Nasution, Olviyani; Lee, Jaok; Srinivasa, Kavitha; Choi, In-Geol; Lee, Young Mi; Kim, Eunjung; Choi, Wonja; Kim, Wankee.

In: Environmental Microbiology, Vol. 17, No. 8, 01.08.2015, p. 2721-2734.

Research output: Contribution to journalArticle

Nasution, Olviyani ; Lee, Jaok ; Srinivasa, Kavitha ; Choi, In-Geol ; Lee, Young Mi ; Kim, Eunjung ; Choi, Wonja ; Kim, Wankee. / Loss of Dfg5 glycosylphosphatidylinositol-anchored membrane protein confers enhanced heat tolerance in Saccharomyces cerevisiae. In: Environmental Microbiology. 2015 ; Vol. 17, No. 8. pp. 2721-2734.
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