Abstract
AIM: To investigate the genomic copy number alterations that may harbor key driver genes in gastric tumorigenesis. METHODS: Using high-resolution array comparative genomic hybridization (CGH), we investigated the genomic alterations of 20 advanced primary gastric adenocarcinomas (seventeen tubular and three mucinous) of Chinese patients from the Jilin province. Ten matching adjacent normal regions from the same patients were also studied. RESULTS: The most frequent imbalances detected in these cancer samples were gains of 3q26.31-q27.2, 5p, 8q, 11p, 18p, 19q and 20q and losses of 3p, 4p, 18q and 21q. The use of high-resolution array CGH increased the resolution and sensitivity of the observed genomic changes and identified focal genetic imbalances, which included 54 gains and 16 losses that were smaller than 1 Mb in size. The most interesting focal imbalances were the intergenic loss/homozygous deletion of the fragile histidine triad gene and the amplicons 11q13, 18q11.2 and 19q12, as well as the novel amplicons 1p36.22 and 11p15.5. CONCLUSION: These regions, especially the focal amplicons, may harbor key driver genes that will serve as biomarkers for either the diagnosis or the prognosis of gastric cancer, and therefore, a large-scale investigation is recommended.
| Original language | English |
|---|---|
| Pages (from-to) | 4522-4532 |
| Number of pages | 11 |
| Journal | World Journal of Gastroenterology |
| Volume | 18 |
| Issue number | 33 |
| DOIs | |
| Publication status | Published - 2012 Oct 26 |
Fingerprint
Keywords
- Amplicon
- Array comparative genomic hybridization
- Fragile histidine triad
- Gastric adenocarcinoma
- Oncogene
ASJC Scopus subject areas
- Gastroenterology
Cite this
Loss of fragile histidine triad and amplification of 1p36.22 and 11p15.5 in primary gastric adenocarcinomas. / Liu, Yuan Yuan; Chen, Hai Ying; Zhang, Man Li; Tian, Dan; Li, Shibo; Lee, Ji-Yun.
In: World Journal of Gastroenterology, Vol. 18, No. 33, 26.10.2012, p. 4522-4532.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Loss of fragile histidine triad and amplification of 1p36.22 and 11p15.5 in primary gastric adenocarcinomas
AU - Liu, Yuan Yuan
AU - Chen, Hai Ying
AU - Zhang, Man Li
AU - Tian, Dan
AU - Li, Shibo
AU - Lee, Ji-Yun
PY - 2012/10/26
Y1 - 2012/10/26
N2 - AIM: To investigate the genomic copy number alterations that may harbor key driver genes in gastric tumorigenesis. METHODS: Using high-resolution array comparative genomic hybridization (CGH), we investigated the genomic alterations of 20 advanced primary gastric adenocarcinomas (seventeen tubular and three mucinous) of Chinese patients from the Jilin province. Ten matching adjacent normal regions from the same patients were also studied. RESULTS: The most frequent imbalances detected in these cancer samples were gains of 3q26.31-q27.2, 5p, 8q, 11p, 18p, 19q and 20q and losses of 3p, 4p, 18q and 21q. The use of high-resolution array CGH increased the resolution and sensitivity of the observed genomic changes and identified focal genetic imbalances, which included 54 gains and 16 losses that were smaller than 1 Mb in size. The most interesting focal imbalances were the intergenic loss/homozygous deletion of the fragile histidine triad gene and the amplicons 11q13, 18q11.2 and 19q12, as well as the novel amplicons 1p36.22 and 11p15.5. CONCLUSION: These regions, especially the focal amplicons, may harbor key driver genes that will serve as biomarkers for either the diagnosis or the prognosis of gastric cancer, and therefore, a large-scale investigation is recommended.
AB - AIM: To investigate the genomic copy number alterations that may harbor key driver genes in gastric tumorigenesis. METHODS: Using high-resolution array comparative genomic hybridization (CGH), we investigated the genomic alterations of 20 advanced primary gastric adenocarcinomas (seventeen tubular and three mucinous) of Chinese patients from the Jilin province. Ten matching adjacent normal regions from the same patients were also studied. RESULTS: The most frequent imbalances detected in these cancer samples were gains of 3q26.31-q27.2, 5p, 8q, 11p, 18p, 19q and 20q and losses of 3p, 4p, 18q and 21q. The use of high-resolution array CGH increased the resolution and sensitivity of the observed genomic changes and identified focal genetic imbalances, which included 54 gains and 16 losses that were smaller than 1 Mb in size. The most interesting focal imbalances were the intergenic loss/homozygous deletion of the fragile histidine triad gene and the amplicons 11q13, 18q11.2 and 19q12, as well as the novel amplicons 1p36.22 and 11p15.5. CONCLUSION: These regions, especially the focal amplicons, may harbor key driver genes that will serve as biomarkers for either the diagnosis or the prognosis of gastric cancer, and therefore, a large-scale investigation is recommended.
KW - Amplicon
KW - Array comparative genomic hybridization
KW - Fragile histidine triad
KW - Gastric adenocarcinoma
KW - Oncogene
UR - http://www.scopus.com/inward/record.url?scp=84867739735&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84867739735&partnerID=8YFLogxK
U2 - 10.3748/wjg.v18.i33.4522
DO - 10.3748/wjg.v18.i33.4522
M3 - Article
VL - 18
SP - 4522
EP - 4532
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
SN - 1007-9327
IS - 33
ER -