Loss of hsp70.1 decreases functional motor recovery after spinal cord injury in mice

Hyun Jeong Kim, Ji In Jung, Youngkyung Kim, Jae Seon Lee, Young Wook Yoon, Junesun Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Heat shock proteins (HSPs) are specifically induced by various forms of stress. Hsp70.1, a member of the hsp70 family is known to play an important role in cytoprotection from stressful insults. However, the functional role of Hsp70 in motor function after spinal cord injury (SCI) is still unclear. To study the role of hsp70.1 in motor recovery following SCI, we assessed locomotor function in hsp70.1 knockout (KO) mice and their wild-type (WT) mice via the Basso, Beattie and Bresnahan (BBB) locomotor rating scale, before and after spinal hemisection at T13 level. We also examined lesion size in the spinal cord using Luxol fast blue/cresyl violet staining. One day after injury, KO and WT mice showed no significant difference in the motor function due to complete paralysis following spinal hemisection. However, when it compared to WT mice, KO mice had significantly delayed and decreased functional outcomes from 4 days up to 21 days after SCI. KO mice also showed significantly greater lesion size in the spinal cord than WT mice showed at 21 days after spinal hemisection. These results suggest that Hsp70 has a protective effect against traumatic SCI and the manipulation of the hsp70.1 gene may help improve the recovery of motor function, thereby enhancing neuroprotection after SCI.

Original languageEnglish
Pages (from-to)157-161
Number of pages5
JournalKorean Journal of Physiology and Pharmacology
Volume14
Issue number3
DOIs
Publication statusPublished - 2010 Jun 1

Fingerprint

Spinal Cord Injuries
Knockout Mice
Spinal Cord
Cytoprotection
Recovery of Function
Heat-Shock Proteins
Paralysis
heat-shock protein 70.1
Staining and Labeling
Wounds and Injuries
Genes

Keywords

  • Heat shock protein
  • Mice
  • Neuroprotection
  • Spinal cord injury

ASJC Scopus subject areas

  • Pharmacology
  • Physiology

Cite this

Loss of hsp70.1 decreases functional motor recovery after spinal cord injury in mice. / Kim, Hyun Jeong; Jung, Ji In; Kim, Youngkyung; Lee, Jae Seon; Yoon, Young Wook; Kim, Junesun.

In: Korean Journal of Physiology and Pharmacology, Vol. 14, No. 3, 01.06.2010, p. 157-161.

Research output: Contribution to journalArticle

Kim, Hyun Jeong ; Jung, Ji In ; Kim, Youngkyung ; Lee, Jae Seon ; Yoon, Young Wook ; Kim, Junesun. / Loss of hsp70.1 decreases functional motor recovery after spinal cord injury in mice. In: Korean Journal of Physiology and Pharmacology. 2010 ; Vol. 14, No. 3. pp. 157-161.
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