Loss of the 29-kilodalton outer membrane protein in the presence of OXA-51-like enzymes in acinetobacter baumannii is associated with decreased imipenem susceptibility

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Abstract

Carbapenem resistance in Acinetobacter baumannii is increasing these days. We investigated the roles of outer membrane proteins and efflux pumps in carbapenem resistance of A. baumannii which showed no carbapenemase activity in modified Hodge test. Among 58 carbapenem-resistant isolates collected from the Korea University Medical Center between January 2002 and March 2006, 17 isolates showed negative results in modified Hodge test. In outer membrane protein analysis, loss of the 29-kDa protein band was related with higher imipenem minimum inhibitory concentrations especially in the presence of OXA-51-like enzymes. Efflux pump-mediated carbapenem resistance was found in one out of the 17 isolates (5.9%). All of the 58 carbapenem-resistant strains and 5 of the 10 carbapenem-susceptible strains had OXA-51-like carbapenemase genes, suggesting that OXA-51-like enzymes may be naturally existing in A. baumannii and have very weak carbapenem hydrolyzing activity.

Original languageEnglish
Pages (from-to)151-158
Number of pages8
JournalMicrobial Drug Resistance
Volume15
Issue number3
DOIs
Publication statusPublished - 2009 Sep 1

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Acinetobacter baumannii
Carbapenems
Imipenem
Membrane Proteins
Enzymes
Microbial Sensitivity Tests
Korea
Asp(5)-oxytocin
Genes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Pharmacology
  • Immunology
  • Microbiology

Cite this

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title = "Loss of the 29-kilodalton outer membrane protein in the presence of OXA-51-like enzymes in acinetobacter baumannii is associated with decreased imipenem susceptibility",
abstract = "Carbapenem resistance in Acinetobacter baumannii is increasing these days. We investigated the roles of outer membrane proteins and efflux pumps in carbapenem resistance of A. baumannii which showed no carbapenemase activity in modified Hodge test. Among 58 carbapenem-resistant isolates collected from the Korea University Medical Center between January 2002 and March 2006, 17 isolates showed negative results in modified Hodge test. In outer membrane protein analysis, loss of the 29-kDa protein band was related with higher imipenem minimum inhibitory concentrations especially in the presence of OXA-51-like enzymes. Efflux pump-mediated carbapenem resistance was found in one out of the 17 isolates (5.9{\%}). All of the 58 carbapenem-resistant strains and 5 of the 10 carbapenem-susceptible strains had OXA-51-like carbapenemase genes, suggesting that OXA-51-like enzymes may be naturally existing in A. baumannii and have very weak carbapenem hydrolyzing activity.",
author = "Jeong, {Hye Won} and Hee-Jin Cheong and Kim, {Woo Joo} and Min, {Ja Kim} and Ki-Joon Song and Song, {Jin Won} and Heenam Kim and Roh, {Kyoung Ho}",
year = "2009",
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language = "English",
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T1 - Loss of the 29-kilodalton outer membrane protein in the presence of OXA-51-like enzymes in acinetobacter baumannii is associated with decreased imipenem susceptibility

AU - Jeong, Hye Won

AU - Cheong, Hee-Jin

AU - Kim, Woo Joo

AU - Min, Ja Kim

AU - Song, Ki-Joon

AU - Song, Jin Won

AU - Kim, Heenam

AU - Roh, Kyoung Ho

PY - 2009/9/1

Y1 - 2009/9/1

N2 - Carbapenem resistance in Acinetobacter baumannii is increasing these days. We investigated the roles of outer membrane proteins and efflux pumps in carbapenem resistance of A. baumannii which showed no carbapenemase activity in modified Hodge test. Among 58 carbapenem-resistant isolates collected from the Korea University Medical Center between January 2002 and March 2006, 17 isolates showed negative results in modified Hodge test. In outer membrane protein analysis, loss of the 29-kDa protein band was related with higher imipenem minimum inhibitory concentrations especially in the presence of OXA-51-like enzymes. Efflux pump-mediated carbapenem resistance was found in one out of the 17 isolates (5.9%). All of the 58 carbapenem-resistant strains and 5 of the 10 carbapenem-susceptible strains had OXA-51-like carbapenemase genes, suggesting that OXA-51-like enzymes may be naturally existing in A. baumannii and have very weak carbapenem hydrolyzing activity.

AB - Carbapenem resistance in Acinetobacter baumannii is increasing these days. We investigated the roles of outer membrane proteins and efflux pumps in carbapenem resistance of A. baumannii which showed no carbapenemase activity in modified Hodge test. Among 58 carbapenem-resistant isolates collected from the Korea University Medical Center between January 2002 and March 2006, 17 isolates showed negative results in modified Hodge test. In outer membrane protein analysis, loss of the 29-kDa protein band was related with higher imipenem minimum inhibitory concentrations especially in the presence of OXA-51-like enzymes. Efflux pump-mediated carbapenem resistance was found in one out of the 17 isolates (5.9%). All of the 58 carbapenem-resistant strains and 5 of the 10 carbapenem-susceptible strains had OXA-51-like carbapenemase genes, suggesting that OXA-51-like enzymes may be naturally existing in A. baumannii and have very weak carbapenem hydrolyzing activity.

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