Lotus-leaf-like structured heparin-conjugated poly(L-lactide-co-e{open}-caprolactone) as a blood compatible material

Jin Ik Lim, Seung il Kim, Soo Hyun Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A heparin-conjugated biodegradable polymer was synthesized by direct coupling of heparin to poly(L-lactide-co-e{open}-caprolactone) (PLCL) and was manufactured into lotus-leaf-like structured films. We evaluated whether lotus-leaf-like structured heparin-conjugated PLCL (LH-PLCL) could be applied to blood vessel tissue engineering. Differences in the surface structures of the films with respect to hydrophobicity and the lotus effect as well as the antithrombotic efficiency in human whole blood were examined using scanning electron microscopy (SEM) and a contact angle meter. Recovery testing was conducted using a tensile strength testing machine, and quantitative analysis of conjugated heparin was performed using the toluidine blue colorimetric method. The concentration of conjugated heparin was 0.14 μg/mg H-PLCL, and the contact angle with the lotus-leaf-like surface was approximately 120°. Furthermore, the LH-PLCL film yielded a lower platelet adhesion rate (around less than 1.4%) in whole blood than that yielded by an untreated PLCL film. These results indicate a unique property of bound heparin and the lotus-leaf-like structure. This novel LH-PLCL polymer could be applied as a blood/tissue compatible biodegradable material for implantable medical devices and tissue engineering.

Original languageEnglish
Pages (from-to)463-467
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Volume103
DOIs
Publication statusPublished - 2013 Mar 1

Fingerprint

heparins
leaves
blood
Heparin
Blood
tissue engineering
Conjugated polymers
Tissue Engineering
Tissue engineering
Contact angle
Polymers
Tolonium Chloride
Biodegradable polymers
poly(lactide)
caprolactone
Tensile Strength
blood vessels
Blood vessels
polymers
Testing

Keywords

  • Antithrombotic material
  • Blood vessel
  • Heparin
  • Lotus-leaf-like structure
  • Surface modification

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Lotus-leaf-like structured heparin-conjugated poly(L-lactide-co-e{open}-caprolactone) as a blood compatible material. / Lim, Jin Ik; Kim, Seung il; Kim, Soo Hyun.

In: Colloids and Surfaces B: Biointerfaces, Vol. 103, 01.03.2013, p. 463-467.

Research output: Contribution to journalArticle

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