Abstract
A heparin-conjugated biodegradable polymer was synthesized by direct coupling of heparin to poly(L-lactide-co-e{open}-caprolactone) (PLCL) and was manufactured into lotus-leaf-like structured films. We evaluated whether lotus-leaf-like structured heparin-conjugated PLCL (LH-PLCL) could be applied to blood vessel tissue engineering. Differences in the surface structures of the films with respect to hydrophobicity and the lotus effect as well as the antithrombotic efficiency in human whole blood were examined using scanning electron microscopy (SEM) and a contact angle meter. Recovery testing was conducted using a tensile strength testing machine, and quantitative analysis of conjugated heparin was performed using the toluidine blue colorimetric method. The concentration of conjugated heparin was 0.14 μg/mg H-PLCL, and the contact angle with the lotus-leaf-like surface was approximately 120°. Furthermore, the LH-PLCL film yielded a lower platelet adhesion rate (around less than 1.4%) in whole blood than that yielded by an untreated PLCL film. These results indicate a unique property of bound heparin and the lotus-leaf-like structure. This novel LH-PLCL polymer could be applied as a blood/tissue compatible biodegradable material for implantable medical devices and tissue engineering.
| Original language | English |
|---|---|
| Pages (from-to) | 463-467 |
| Number of pages | 5 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 103 |
| DOIs | |
| Publication status | Published - 2013 Mar 1 |
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Keywords
- Antithrombotic material
- Blood vessel
- Heparin
- Lotus-leaf-like structure
- Surface modification
ASJC Scopus subject areas
- Biotechnology
- Colloid and Surface Chemistry
- Physical and Theoretical Chemistry
- Surfaces and Interfaces
Cite this
Lotus-leaf-like structured heparin-conjugated poly(L-lactide-co-e{open}-caprolactone) as a blood compatible material. / Lim, Jin Ik; Kim, Seung il; Kim, Soo Hyun.
In: Colloids and Surfaces B: Biointerfaces, Vol. 103, 01.03.2013, p. 463-467.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lotus-leaf-like structured heparin-conjugated poly(L-lactide-co-e{open}-caprolactone) as a blood compatible material
AU - Lim, Jin Ik
AU - Kim, Seung il
AU - Kim, Soo Hyun
PY - 2013/3/1
Y1 - 2013/3/1
N2 - A heparin-conjugated biodegradable polymer was synthesized by direct coupling of heparin to poly(L-lactide-co-e{open}-caprolactone) (PLCL) and was manufactured into lotus-leaf-like structured films. We evaluated whether lotus-leaf-like structured heparin-conjugated PLCL (LH-PLCL) could be applied to blood vessel tissue engineering. Differences in the surface structures of the films with respect to hydrophobicity and the lotus effect as well as the antithrombotic efficiency in human whole blood were examined using scanning electron microscopy (SEM) and a contact angle meter. Recovery testing was conducted using a tensile strength testing machine, and quantitative analysis of conjugated heparin was performed using the toluidine blue colorimetric method. The concentration of conjugated heparin was 0.14 μg/mg H-PLCL, and the contact angle with the lotus-leaf-like surface was approximately 120°. Furthermore, the LH-PLCL film yielded a lower platelet adhesion rate (around less than 1.4%) in whole blood than that yielded by an untreated PLCL film. These results indicate a unique property of bound heparin and the lotus-leaf-like structure. This novel LH-PLCL polymer could be applied as a blood/tissue compatible biodegradable material for implantable medical devices and tissue engineering.
AB - A heparin-conjugated biodegradable polymer was synthesized by direct coupling of heparin to poly(L-lactide-co-e{open}-caprolactone) (PLCL) and was manufactured into lotus-leaf-like structured films. We evaluated whether lotus-leaf-like structured heparin-conjugated PLCL (LH-PLCL) could be applied to blood vessel tissue engineering. Differences in the surface structures of the films with respect to hydrophobicity and the lotus effect as well as the antithrombotic efficiency in human whole blood were examined using scanning electron microscopy (SEM) and a contact angle meter. Recovery testing was conducted using a tensile strength testing machine, and quantitative analysis of conjugated heparin was performed using the toluidine blue colorimetric method. The concentration of conjugated heparin was 0.14 μg/mg H-PLCL, and the contact angle with the lotus-leaf-like surface was approximately 120°. Furthermore, the LH-PLCL film yielded a lower platelet adhesion rate (around less than 1.4%) in whole blood than that yielded by an untreated PLCL film. These results indicate a unique property of bound heparin and the lotus-leaf-like structure. This novel LH-PLCL polymer could be applied as a blood/tissue compatible biodegradable material for implantable medical devices and tissue engineering.
KW - Antithrombotic material
KW - Blood vessel
KW - Heparin
KW - Lotus-leaf-like structure
KW - Surface modification
UR - http://www.scopus.com/inward/record.url?scp=84871169402&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84871169402&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2012.11.016
DO - 10.1016/j.colsurfb.2012.11.016
M3 - Article
C2 - 23261567
AN - SCOPUS:84871169402
VL - 103
SP - 463
EP - 467
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -