TY - JOUR
T1 - Low-blinking SERS substrate for switchable detection of kanamycin
AU - Nguyen, Anh H.
AU - Ma, Xingyi
AU - Park, Hyun Gyu
AU - Sim, Sang Jun
N1 - Funding Information:
This study was supported by the National Research Foundation (NRF) grant ( 2016R1A2A1A05005465/2010–0027955/2018R1A2A1A05022355 ) and the grants ( 2014M1A8A1049278 ) from Korea Carbon Capture and Sequestration (CCS) R&D Centre of the National Research Foundation (NRF) of the Ministry of Science, ICT, and Future Planning of Korea . X.M. was supported by a Research Professor program ( 141594 ) of Korea University.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - Metallic plasmonic nanoparticles generate surface-enhanced Raman Scattering (SERS) for wide sensing applications of biomolecules, gas and environmental contaminants. However, the metallic materials cause high thermal radiation during laser excitation in the opto-microfluidic systems and give rise to temporal intensity fluctuations, which is called blinking effects in SERS spectra. This work developed nanocomposite consisting of graphene oxide (GO) and gold nanoparticles (AuNPs) with high thermal stability and low thermal radiation, which provided plasmonic resonance while reduced blinking effect for SERS-based sensing in the microfluidic detection system. The system was applied to detect kanamycin in drinking water, orange juice, and milk by using Texas Red-dye conjugated Beacon aptamer, where the aptamer was designed to be specific and switchable in kanamycin recognition. The sensor was reusable, and its limit of detection was 0.75 nM in a linear range of 1 nM to 100 nM. The microfluidic SERS detection system can be potentially used for sensitive and reliable assays of small molecules.
AB - Metallic plasmonic nanoparticles generate surface-enhanced Raman Scattering (SERS) for wide sensing applications of biomolecules, gas and environmental contaminants. However, the metallic materials cause high thermal radiation during laser excitation in the opto-microfluidic systems and give rise to temporal intensity fluctuations, which is called blinking effects in SERS spectra. This work developed nanocomposite consisting of graphene oxide (GO) and gold nanoparticles (AuNPs) with high thermal stability and low thermal radiation, which provided plasmonic resonance while reduced blinking effect for SERS-based sensing in the microfluidic detection system. The system was applied to detect kanamycin in drinking water, orange juice, and milk by using Texas Red-dye conjugated Beacon aptamer, where the aptamer was designed to be specific and switchable in kanamycin recognition. The sensor was reusable, and its limit of detection was 0.75 nM in a linear range of 1 nM to 100 nM. The microfluidic SERS detection system can be potentially used for sensitive and reliable assays of small molecules.
KW - Antibiotic sensor
KW - Blinking effects
KW - Gold nanoparticles
KW - Graphene oxide
KW - SERS
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U2 - 10.1016/j.snb.2018.11.037
DO - 10.1016/j.snb.2018.11.037
M3 - Article
AN - SCOPUS:85057480079
VL - 282
SP - 765
EP - 773
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
ER -