Abstract
Metallic plasmonic nanoparticles generate surface-enhanced Raman Scattering (SERS) for wide sensing applications of biomolecules, gas and environmental contaminants. However, the metallic materials cause high thermal radiation during laser excitation in the opto-microfluidic systems and give rise to temporal intensity fluctuations, which is called blinking effects in SERS spectra. This work developed nanocomposite consisting of graphene oxide (GO) and gold nanoparticles (AuNPs) with high thermal stability and low thermal radiation, which provided plasmonic resonance while reduced blinking effect for SERS-based sensing in the microfluidic detection system. The system was applied to detect kanamycin in drinking water, orange juice, and milk by using Texas Red-dye conjugated Beacon aptamer, where the aptamer was designed to be specific and switchable in kanamycin recognition. The sensor was reusable, and its limit of detection was 0.75 nM in a linear range of 1 nM to 100 nM. The microfluidic SERS detection system can be potentially used for sensitive and reliable assays of small molecules.
| Original language | English |
|---|---|
| Pages (from-to) | 765-773 |
| Number of pages | 9 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 282 |
| DOIs | |
| Publication status | Published - 2019 Mar 1 |
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Keywords
- Antibiotic sensor
- Blinking effects
- Gold nanoparticles
- Graphene oxide
- SERS
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Instrumentation
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry
Cite this
Low-blinking SERS substrate for switchable detection of kanamycin. / Nguyen, Anh H.; Ma, Xingyi; Park, Hyun Gyu; Sim, Sang Jun.
In: Sensors and Actuators, B: Chemical, Vol. 282, 01.03.2019, p. 765-773.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Low-blinking SERS substrate for switchable detection of kanamycin
AU - Nguyen, Anh H.
AU - Ma, Xingyi
AU - Park, Hyun Gyu
AU - Sim, Sang Jun
PY - 2019/3/1
Y1 - 2019/3/1
N2 - Metallic plasmonic nanoparticles generate surface-enhanced Raman Scattering (SERS) for wide sensing applications of biomolecules, gas and environmental contaminants. However, the metallic materials cause high thermal radiation during laser excitation in the opto-microfluidic systems and give rise to temporal intensity fluctuations, which is called blinking effects in SERS spectra. This work developed nanocomposite consisting of graphene oxide (GO) and gold nanoparticles (AuNPs) with high thermal stability and low thermal radiation, which provided plasmonic resonance while reduced blinking effect for SERS-based sensing in the microfluidic detection system. The system was applied to detect kanamycin in drinking water, orange juice, and milk by using Texas Red-dye conjugated Beacon aptamer, where the aptamer was designed to be specific and switchable in kanamycin recognition. The sensor was reusable, and its limit of detection was 0.75 nM in a linear range of 1 nM to 100 nM. The microfluidic SERS detection system can be potentially used for sensitive and reliable assays of small molecules.
AB - Metallic plasmonic nanoparticles generate surface-enhanced Raman Scattering (SERS) for wide sensing applications of biomolecules, gas and environmental contaminants. However, the metallic materials cause high thermal radiation during laser excitation in the opto-microfluidic systems and give rise to temporal intensity fluctuations, which is called blinking effects in SERS spectra. This work developed nanocomposite consisting of graphene oxide (GO) and gold nanoparticles (AuNPs) with high thermal stability and low thermal radiation, which provided plasmonic resonance while reduced blinking effect for SERS-based sensing in the microfluidic detection system. The system was applied to detect kanamycin in drinking water, orange juice, and milk by using Texas Red-dye conjugated Beacon aptamer, where the aptamer was designed to be specific and switchable in kanamycin recognition. The sensor was reusable, and its limit of detection was 0.75 nM in a linear range of 1 nM to 100 nM. The microfluidic SERS detection system can be potentially used for sensitive and reliable assays of small molecules.
KW - Antibiotic sensor
KW - Blinking effects
KW - Gold nanoparticles
KW - Graphene oxide
KW - SERS
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U2 - 10.1016/j.snb.2018.11.037
DO - 10.1016/j.snb.2018.11.037
M3 - Article
AN - SCOPUS:85057480079
VL - 282
SP - 765
EP - 773
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
ER -