LPLUNC1 modulates innate immune responses to vibrio cholerae

Ok Shin, Taher Uddin, Robert Citorik, Jennifer P. Wang, Patricia Della Pelle, Richard L. Kradin, Colin D. Bingle, Lynne Bingle, Andrew Camilli, Taufiqur R. Bhuiyan, Tahmina Shirin, Edward T. Ryan, Stephen B. Calderwood, Robert W. Finberg, Firdausi Qadri, Regina C. Larocque, Jason B. Harris

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background. Recent studies demonstrate that long palate, lung, and nasal epithelium clone 1 protein (LPLUNC1) is involved in immune responses to Vibrio cholerae, and that variations in the LPLUNC1 promoter influence susceptibility to severe cholera in humans. However, no functional role for LPLUNC1 has been identified.Metods.We investigated the role of LPLUNC1 in immune responses to V. cholerae, assessing its affect on bacterial growth and killing and on innate inflammatory responses to bacterial outer membrane components, including purified lipopolysaccharide (LPS) and outer membrane vesicles. We performed immunostaining for LPLUNC1 in duodenal biopsies from cholera patients and uninfected controls.Results.LPLUNC1 decreased proinflammatory innate immune responses to V. cholerae and Escherichia coli LPS. The effect of LPLUNC1 was dose-dependent and occurred in a TLR4-dependent manner. LPLUNC1 did not affect lipoprotein-mediated TLR2 activation. Immunostaining demonstrated expression of LPLUNC1 in Paneth cells in cholera patients and controls.Conclusions.Our results demonstrate that LPLUNC1 is expressed in Paneth cells and likely plays a role in modulating host inflammatory responses to V. cholerae infection. Attenuation of innate immune responses to LPS by LPLUNC1 may have implications for the maintenance of immune homeostasis in the intestine.

Original languageEnglish
Pages (from-to)1349-1357
Number of pages9
JournalJournal of Infectious Diseases
Volume204
Issue number9
DOIs
Publication statusPublished - 2011 Nov 1
Externally publishedYes

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Vibrio cholerae
Nasal Mucosa
Palate
Innate Immunity
Clone Cells
Lung
Proteins
Cholera
Paneth Cells
Lipopolysaccharides
Vibrio Infections
Membranes
Lipoproteins
Intestines
Homeostasis
Maintenance
Escherichia coli
Biopsy

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Shin, O., Uddin, T., Citorik, R., Wang, J. P., Della Pelle, P., Kradin, R. L., ... Harris, J. B. (2011). LPLUNC1 modulates innate immune responses to vibrio cholerae. Journal of Infectious Diseases, 204(9), 1349-1357. https://doi.org/10.1093/infdis/jir544

LPLUNC1 modulates innate immune responses to vibrio cholerae. / Shin, Ok; Uddin, Taher; Citorik, Robert; Wang, Jennifer P.; Della Pelle, Patricia; Kradin, Richard L.; Bingle, Colin D.; Bingle, Lynne; Camilli, Andrew; Bhuiyan, Taufiqur R.; Shirin, Tahmina; Ryan, Edward T.; Calderwood, Stephen B.; Finberg, Robert W.; Qadri, Firdausi; Larocque, Regina C.; Harris, Jason B.

In: Journal of Infectious Diseases, Vol. 204, No. 9, 01.11.2011, p. 1349-1357.

Research output: Contribution to journalArticle

Shin, O, Uddin, T, Citorik, R, Wang, JP, Della Pelle, P, Kradin, RL, Bingle, CD, Bingle, L, Camilli, A, Bhuiyan, TR, Shirin, T, Ryan, ET, Calderwood, SB, Finberg, RW, Qadri, F, Larocque, RC & Harris, JB 2011, 'LPLUNC1 modulates innate immune responses to vibrio cholerae', Journal of Infectious Diseases, vol. 204, no. 9, pp. 1349-1357. https://doi.org/10.1093/infdis/jir544
Shin O, Uddin T, Citorik R, Wang JP, Della Pelle P, Kradin RL et al. LPLUNC1 modulates innate immune responses to vibrio cholerae. Journal of Infectious Diseases. 2011 Nov 1;204(9):1349-1357. https://doi.org/10.1093/infdis/jir544
Shin, Ok ; Uddin, Taher ; Citorik, Robert ; Wang, Jennifer P. ; Della Pelle, Patricia ; Kradin, Richard L. ; Bingle, Colin D. ; Bingle, Lynne ; Camilli, Andrew ; Bhuiyan, Taufiqur R. ; Shirin, Tahmina ; Ryan, Edward T. ; Calderwood, Stephen B. ; Finberg, Robert W. ; Qadri, Firdausi ; Larocque, Regina C. ; Harris, Jason B. / LPLUNC1 modulates innate immune responses to vibrio cholerae. In: Journal of Infectious Diseases. 2011 ; Vol. 204, No. 9. pp. 1349-1357.
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abstract = "Background. Recent studies demonstrate that long palate, lung, and nasal epithelium clone 1 protein (LPLUNC1) is involved in immune responses to Vibrio cholerae, and that variations in the LPLUNC1 promoter influence susceptibility to severe cholera in humans. However, no functional role for LPLUNC1 has been identified.Metods.We investigated the role of LPLUNC1 in immune responses to V. cholerae, assessing its affect on bacterial growth and killing and on innate inflammatory responses to bacterial outer membrane components, including purified lipopolysaccharide (LPS) and outer membrane vesicles. We performed immunostaining for LPLUNC1 in duodenal biopsies from cholera patients and uninfected controls.Results.LPLUNC1 decreased proinflammatory innate immune responses to V. cholerae and Escherichia coli LPS. The effect of LPLUNC1 was dose-dependent and occurred in a TLR4-dependent manner. LPLUNC1 did not affect lipoprotein-mediated TLR2 activation. Immunostaining demonstrated expression of LPLUNC1 in Paneth cells in cholera patients and controls.Conclusions.Our results demonstrate that LPLUNC1 is expressed in Paneth cells and likely plays a role in modulating host inflammatory responses to V. cholerae infection. Attenuation of innate immune responses to LPS by LPLUNC1 may have implications for the maintenance of immune homeostasis in the intestine.",
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AU - Shin, Ok

AU - Uddin, Taher

AU - Citorik, Robert

AU - Wang, Jennifer P.

AU - Della Pelle, Patricia

AU - Kradin, Richard L.

AU - Bingle, Colin D.

AU - Bingle, Lynne

AU - Camilli, Andrew

AU - Bhuiyan, Taufiqur R.

AU - Shirin, Tahmina

AU - Ryan, Edward T.

AU - Calderwood, Stephen B.

AU - Finberg, Robert W.

AU - Qadri, Firdausi

AU - Larocque, Regina C.

AU - Harris, Jason B.

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