Lysophosphatidic acid (LPA) receptor 3-mediated LPA signal transduction pathways: A possible relationship with early development of peri-implantation porcine conceptus

Wooyoung Jeong, Heewon Seo, Yujin Sung, Hakhyun Ka, Gwonhwa Song, Jinyoung Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Lysophosphatidic acid (LPA) is a phospholipid with a variety of fatty acyl groups that mediates diverse biological effects on various types of cells through specific G protein-coupled receptors. LPA appears to play a significant role in many reproductive processes, including luteolysis, implantation, and placentation. Our previous study in pigs demonstrated that LPA and the LPA receptor system are present at the maternalconceptus interface and that LPA increases uterine endometrial expression of prostaglandin-endoperoxide synthase 2 (PTGS2) through LPA receptor 3 (LPAR3). However, the role of LPA in conceptuses during early pregnancy has not been determined. Therefore, this study examined the effects of LPA in cell proliferation, migration, and activation of the intracellular signaling pathway in porcine conceptuses by using an established porcine trophectoderm (pTr) cell line isolated from Day 12 conceptuses. All examined LPA species with various fatty acid lengths increased proliferation and migration of pTr cells as the dosage increased. Immunoblot analyses found that LPA activated intracellular signaling molecules, extracellular signal-regulated kinase 1/2 (ERK1/2), ribosomal protein S6 kinase 90 kDa (P90RSK), ribosomal protein S6 (RPS6), and P38 in pTr cells. Furthermore, LPA increased expression of PTGS2 and urokinasetype plasminogen activator (PLAU), and the LPA-induced increases in PTGS2 and PLAU expression were inhibited by LPAR3 siRNA. Collectively, these results showed that LPA promotes proliferation, migration, and differentiation of pTr cells by activating the ERK1/2-P90RSK-RPS6 and P38 pathways, indicating that the LPA-LPAR3 system may be involved in the development of trophoblast during early pregnancy in pigs.

Original languageEnglish
Article number104
JournalBiology of Reproduction
Volume94
Issue number5
DOIs
Publication statusPublished - 2016 May 1

Fingerprint

Lysophosphatidic Acid Receptors
Signal Transduction
Swine
Prostaglandin-Endoperoxide Synthases
Ribosomal Protein S6
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
90-kDa Ribosomal Protein S6 Kinases
lysophosphatidic acid
Luteolysis
Placentation
Pregnancy
Plasminogen Activators
Trophoblasts
G-Protein-Coupled Receptors
Small Interfering RNA
Cell Movement
Phospholipids

Keywords

  • Conceptus
  • Lysophosphatidic acid
  • Migration
  • Pig
  • Proliferation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Lysophosphatidic acid (LPA) receptor 3-mediated LPA signal transduction pathways : A possible relationship with early development of peri-implantation porcine conceptus. / Jeong, Wooyoung; Seo, Heewon; Sung, Yujin; Ka, Hakhyun; Song, Gwonhwa; Kim, Jinyoung.

In: Biology of Reproduction, Vol. 94, No. 5, 104, 01.05.2016.

Research output: Contribution to journalArticle

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