Lysophosphatidylcholine up-regulates CXCR4 chemokine receptor expression in human CD4 T cells

Ki Hoon Han, Kyung Hee Hong, Jesang Ko, Kyong Suk Rhee, Myeong Ki Hong, Jae Joong Kim, You Ho Kim, Seung Jung Park

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Oxidized low-density lipoprotein (OxLDL) is an inflammatory modulator in the atherosclerotic plaque. We examined the effect of lysophosphatidyleholine (lysoPC), a main phospholipid component of OxLDL, on inflammatory responses in human CD4 T cells. We found that lysoPC dose- and time-dependently increased expression of CXCR4, the chemokine receptor on CD4 T cells. This increase was inhibited by caffeic acid phenethyl ester or SN50, nuclear factor-κB inhibitors, and also by suppression of G2A expression, the specific receptor for lysoPC, using antisense oligonucleotide. lysoPC enhanced CD4 T cell chemotaxis in response to stromal cell-derived factor-1 (SDF-1), the exclusive ligand for CXCR4. lysoPC also enhanced SDF-1-stimulated production of inflammatory cytokines interleukin-2 and interferon-γ by CD4 T cells activated by anti-CD3 immunoglobulin G. In conclusion, this study demonstrates that lysoPC directly modulates inflammatory responses in human CD4 T cells. The data suggest that the presence of lysoPC and SDF-1 in atherosclerotic lesions may trigger inflammatory responses mediated by CD4 T cells, which may play an important role in progression of atherosclerosis.

Original languageEnglish
Pages (from-to)195-202
Number of pages8
JournalJournal of Leukocyte Biology
Volume76
Issue number1
DOIs
Publication statusPublished - 2004 Jul 1

    Fingerprint

Keywords

  • Atherosclerosis
  • CD4
  • CD4 T cells
  • CXCR4
  • Lysophosphatidyl-choline
  • SDF-1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

Cite this

Han, K. H., Hong, K. H., Ko, J., Rhee, K. S., Hong, M. K., Kim, J. J., Kim, Y. H., & Park, S. J. (2004). Lysophosphatidylcholine up-regulates CXCR4 chemokine receptor expression in human CD4 T cells. Journal of Leukocyte Biology, 76(1), 195-202. https://doi.org/10.1189/jlb.1103563