Maclurin suppresses migration and invasion of human non-small-cell lung cancer cells via anti-oxidative activity and inhibition of the Src/FAK–ERK–β-catenin pathway

Min Jung Ku, Ji Hyun Kim, Jongsung Lee, Jae Youl Cho, Taehoon Chun, Sang Yeol Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Recent reports indicated that ROS is closely related with cancer metastasis. ROS targets major signaling molecules which are known to be involved in migration and invasion of cancer cells. Here we report that maclurin, a major phenolic component of ethanol extracted mulberry twigs, exerts anti-metastatic effect in A549 human non-small-cell lung cancer cells. Maclurin suppresses intracellular ROS level in A549 human non-small-cell lung cancer cells. Also, maclurin down-regulates Src and ERK, which are well known to be regulated with redox state. Suppressed Src/FAK and ERK signalings activate GSK3-β, thus inhibiting nuclear accumulation of β-catenin. As a result, transcriptional expressions of two major gelatinases (MMP-2 and MMP-9) were significantly down-regulated. Consequently, migration and invasion of A549 human non-small-cell lung cancer cells were attenuated. Anti-metastatic effect of maclurin on A549 human non-small-cell lung cancer cells were diminished by the treatment of hydrogen peroxide, thus further implicating that the effect of maclurin may be strongly related with its anti-oxidative activity. Thus, our data indicate that the anti-metastatic effect of maclurin is exerted by anti-oxidative activity and inhibition of Src/FAK–ERK–β-catenin signaling pathway.

Original languageEnglish
Pages (from-to)243-252
Number of pages10
JournalMolecular and Cellular Biochemistry
Volume402
Issue number1-2
DOIs
Publication statusPublished - 2015

Fingerprint

Catenins
Non-Small Cell Lung Carcinoma
Cells
Matrix Metalloproteinases
Morus
Gelatinases
Hydrogen Peroxide
Oxidation-Reduction
maclurin
Neoplasms
Ethanol
Down-Regulation
Neoplasm Metastasis
Molecules

Keywords

  • Maclurin
  • Matrix metalloproteinases
  • Metastasis
  • Non-small-cell lung cancer
  • Reactive oxygen species

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Maclurin suppresses migration and invasion of human non-small-cell lung cancer cells via anti-oxidative activity and inhibition of the Src/FAK–ERK–β-catenin pathway. / Ku, Min Jung; Kim, Ji Hyun; Lee, Jongsung; Cho, Jae Youl; Chun, Taehoon; Lee, Sang Yeol.

In: Molecular and Cellular Biochemistry, Vol. 402, No. 1-2, 2015, p. 243-252.

Research output: Contribution to journalArticle

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AB - Recent reports indicated that ROS is closely related with cancer metastasis. ROS targets major signaling molecules which are known to be involved in migration and invasion of cancer cells. Here we report that maclurin, a major phenolic component of ethanol extracted mulberry twigs, exerts anti-metastatic effect in A549 human non-small-cell lung cancer cells. Maclurin suppresses intracellular ROS level in A549 human non-small-cell lung cancer cells. Also, maclurin down-regulates Src and ERK, which are well known to be regulated with redox state. Suppressed Src/FAK and ERK signalings activate GSK3-β, thus inhibiting nuclear accumulation of β-catenin. As a result, transcriptional expressions of two major gelatinases (MMP-2 and MMP-9) were significantly down-regulated. Consequently, migration and invasion of A549 human non-small-cell lung cancer cells were attenuated. Anti-metastatic effect of maclurin on A549 human non-small-cell lung cancer cells were diminished by the treatment of hydrogen peroxide, thus further implicating that the effect of maclurin may be strongly related with its anti-oxidative activity. Thus, our data indicate that the anti-metastatic effect of maclurin is exerted by anti-oxidative activity and inhibition of Src/FAK–ERK–β-catenin signaling pathway.

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