Macromolecule delivery into hard-to-transefct primary cells via hydrodynamic cell deformation

Jeongsoo Hur, Aram Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a versatile microfluidic intracellular delivery platform that can deliver large nanomaterials effectively into diverse primary cells via hydrodynamic cell deformation in a novel T-junction microchannel with a cavity. Briefly, a syringe pump was used to inject a cell suspension with cargo of interest into a microchannel at a moderate Re to induce the development of inertial vortices. The recirculating flows deformed the cells, generating transient nanopores on the cellular membrane. This intrinsically cell deformation enables highly effective transport of different nanomaterials into various cell types including difficult-to-transfect primary cells, in a high-throughput and minimally invasive manner.

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages817-818
Number of pages2
ISBN (Electronic)9781733419017
Publication statusPublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: 2020 Oct 42020 Oct 9

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period20/10/420/10/9

Keywords

  • Cell Transfection
  • Cell-based Therapy
  • Gene Delivery
  • Hydroporator
  • Intracellular delivery

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering
  • Chemistry(all)
  • Control and Systems Engineering

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