TY - GEN
T1 - Macromolecule delivery into hard-to-transefct primary cells via hydrodynamic cell deformation
AU - Hur, Jeongsoo
AU - Chung, Aram
N1 - Funding Information:
This work is supported by the Samsung Research Funding and Incubation Center for Future Technology (Grant No. SRFC-IT1802-03).
PY - 2020
Y1 - 2020
N2 - We present a versatile microfluidic intracellular delivery platform that can deliver large nanomaterials effectively into diverse primary cells via hydrodynamic cell deformation in a novel T-junction microchannel with a cavity. Briefly, a syringe pump was used to inject a cell suspension with cargo of interest into a microchannel at a moderate Re to induce the development of inertial vortices. The recirculating flows deformed the cells, generating transient nanopores on the cellular membrane. This intrinsically cell deformation enables highly effective transport of different nanomaterials into various cell types including difficult-to-transfect primary cells, in a high-throughput and minimally invasive manner.
AB - We present a versatile microfluidic intracellular delivery platform that can deliver large nanomaterials effectively into diverse primary cells via hydrodynamic cell deformation in a novel T-junction microchannel with a cavity. Briefly, a syringe pump was used to inject a cell suspension with cargo of interest into a microchannel at a moderate Re to induce the development of inertial vortices. The recirculating flows deformed the cells, generating transient nanopores on the cellular membrane. This intrinsically cell deformation enables highly effective transport of different nanomaterials into various cell types including difficult-to-transfect primary cells, in a high-throughput and minimally invasive manner.
KW - Cell Transfection
KW - Cell-based Therapy
KW - Gene Delivery
KW - Hydroporator
KW - Intracellular delivery
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M3 - Conference contribution
AN - SCOPUS:85098282970
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 817
EP - 818
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -