Macrophage stimulating activity of exo-biopolymer from submerged culture of Lentinus edodes with rice bran

Kwang Won Yu, Kwang Soon Shin, Yang Mun Choi, Hyung Joo Suh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To find a new utilization of rice bran, nine higher fungi were examined for the production of exo-biopolymer with macrophage stimulating activity from rice bran. Among the exo-biopolymers produced from submerged cultures, Lentinus edodes showed the highest activity, followed by Grifola frondosa, Schizophyllum commune, and Coriolus versicolor. L. edodes also had the most potent macrophage stimulating activity in a liquid culture rather than in a solid culture. In order to improve rice bran utilization and the yield of exo-biopolymer with macrophage stimulating activity, the treatment of Rapidase effectively increased the macrophage stimulating activity (about 30% increase), whereas the other enzymes (Econase, Viscozyme, Ultraflo, Celluclast, and Thermylase) treatments did not increase the macrophage stimulating activity. Exo-biopolymer with macrophage stimulating activity from L. edodes contained mainly neutral sugars (58.7%) with considerable amounts of uronic acid (32.2%) and a small amount of proteins (9.1%). Component sugars of exo-biopolymer consisted of mainly arabinose, galactose, glucose, mannose, and xylose (0.95:0.81:0.96:1.00: 0.39, respectively). When the exo-biopolymer was treated with NaIO4, NaClO2, and pronase, the NaClO2 treatment and pronase digestion had little effect, whereas NaIO4 oxidation significantly decreased the macrophage stimulating activity (47.6% reduction at 100 μg/ml). Therefore, the carbohydrate moiety in exo-biopolymer from L. edodes plays an important role in the expression of the macrophage stimulating activity.

Original languageEnglish
Pages (from-to)658-664
Number of pages7
JournalJournal of microbiology and biotechnology
Volume14
Issue number4
Publication statusPublished - 2004 Aug

Keywords

  • Exo-biopolymer
  • Lentinus edodes
  • Macrophage stimulating activity
  • Submerged culture

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Macrophage stimulating activity of exo-biopolymer from submerged culture of Lentinus edodes with rice bran'. Together they form a unique fingerprint.

  • Cite this