Macrophage stimulating activity of exo-biopolymer from submerged culture of Lentinus edodes with rice bran

Kwang Won Yu, Kwang Soon Shin, Yang Mun Choi, Hyung Joo Suh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To find a new utilization of rice bran, nine higher fungi were examined for the production of exo-biopolymer with macrophage stimulating activity from rice bran. Among the exo-biopolymers produced from submerged cultures, Lentinus edodes showed the highest activity, followed by Grifola frondosa, Schizophyllum commune, and Coriolus versicolor. L. edodes also had the most potent macrophage stimulating activity in a liquid culture rather than in a solid culture. In order to improve rice bran utilization and the yield of exo-biopolymer with macrophage stimulating activity, the treatment of Rapidase effectively increased the macrophage stimulating activity (about 30% increase), whereas the other enzymes (Econase, Viscozyme, Ultraflo, Celluclast, and Thermylase) treatments did not increase the macrophage stimulating activity. Exo-biopolymer with macrophage stimulating activity from L. edodes contained mainly neutral sugars (58.7%) with considerable amounts of uronic acid (32.2%) and a small amount of proteins (9.1%). Component sugars of exo-biopolymer consisted of mainly arabinose, galactose, glucose, mannose, and xylose (0.95:0.81:0.96:1.00: 0.39, respectively). When the exo-biopolymer was treated with NaIO4, NaClO2, and pronase, the NaClO2 treatment and pronase digestion had little effect, whereas NaIO4 oxidation significantly decreased the macrophage stimulating activity (47.6% reduction at 100 μg/ml). Therefore, the carbohydrate moiety in exo-biopolymer from L. edodes plays an important role in the expression of the macrophage stimulating activity.

Original languageEnglish
Pages (from-to)658-664
Number of pages7
JournalJournal of Microbiology and Biotechnology
Volume14
Issue number4
Publication statusPublished - 2004 Aug 1

Fingerprint

Shiitake Mushrooms
Biopolymers
Macrophages
Cell culture
Pronase
Sugars
Grifola
Schizophyllum
Uronic Acids
Arabinose
Xylose
Oryza
Carbohydrates
Mannose
Fungi
Galactose
Glucose
Digestion
Thermodynamic properties
Enzymes

Keywords

  • Exo-biopolymer
  • Lentinus edodes
  • Macrophage stimulating activity
  • Submerged culture

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Macrophage stimulating activity of exo-biopolymer from submerged culture of Lentinus edodes with rice bran. / Yu, Kwang Won; Shin, Kwang Soon; Choi, Yang Mun; Suh, Hyung Joo.

In: Journal of Microbiology and Biotechnology, Vol. 14, No. 4, 01.08.2004, p. 658-664.

Research output: Contribution to journalArticle

@article{eed2cf147f2e4784b090cb6e5404f4e3,
title = "Macrophage stimulating activity of exo-biopolymer from submerged culture of Lentinus edodes with rice bran",
abstract = "To find a new utilization of rice bran, nine higher fungi were examined for the production of exo-biopolymer with macrophage stimulating activity from rice bran. Among the exo-biopolymers produced from submerged cultures, Lentinus edodes showed the highest activity, followed by Grifola frondosa, Schizophyllum commune, and Coriolus versicolor. L. edodes also had the most potent macrophage stimulating activity in a liquid culture rather than in a solid culture. In order to improve rice bran utilization and the yield of exo-biopolymer with macrophage stimulating activity, the treatment of Rapidase effectively increased the macrophage stimulating activity (about 30{\%} increase), whereas the other enzymes (Econase, Viscozyme, Ultraflo, Celluclast, and Thermylase) treatments did not increase the macrophage stimulating activity. Exo-biopolymer with macrophage stimulating activity from L. edodes contained mainly neutral sugars (58.7{\%}) with considerable amounts of uronic acid (32.2{\%}) and a small amount of proteins (9.1{\%}). Component sugars of exo-biopolymer consisted of mainly arabinose, galactose, glucose, mannose, and xylose (0.95:0.81:0.96:1.00: 0.39, respectively). When the exo-biopolymer was treated with NaIO4, NaClO2, and pronase, the NaClO2 treatment and pronase digestion had little effect, whereas NaIO4 oxidation significantly decreased the macrophage stimulating activity (47.6{\%} reduction at 100 μg/ml). Therefore, the carbohydrate moiety in exo-biopolymer from L. edodes plays an important role in the expression of the macrophage stimulating activity.",
keywords = "Exo-biopolymer, Lentinus edodes, Macrophage stimulating activity, Submerged culture",
author = "Yu, {Kwang Won} and Shin, {Kwang Soon} and Choi, {Yang Mun} and Suh, {Hyung Joo}",
year = "2004",
month = "8",
day = "1",
language = "English",
volume = "14",
pages = "658--664",
journal = "Journal of Microbiology and Biotechnology",
issn = "1017-7825",
publisher = "Korean Society for Microbiolog and Biotechnology",
number = "4",

}

TY - JOUR

T1 - Macrophage stimulating activity of exo-biopolymer from submerged culture of Lentinus edodes with rice bran

AU - Yu, Kwang Won

AU - Shin, Kwang Soon

AU - Choi, Yang Mun

AU - Suh, Hyung Joo

PY - 2004/8/1

Y1 - 2004/8/1

N2 - To find a new utilization of rice bran, nine higher fungi were examined for the production of exo-biopolymer with macrophage stimulating activity from rice bran. Among the exo-biopolymers produced from submerged cultures, Lentinus edodes showed the highest activity, followed by Grifola frondosa, Schizophyllum commune, and Coriolus versicolor. L. edodes also had the most potent macrophage stimulating activity in a liquid culture rather than in a solid culture. In order to improve rice bran utilization and the yield of exo-biopolymer with macrophage stimulating activity, the treatment of Rapidase effectively increased the macrophage stimulating activity (about 30% increase), whereas the other enzymes (Econase, Viscozyme, Ultraflo, Celluclast, and Thermylase) treatments did not increase the macrophage stimulating activity. Exo-biopolymer with macrophage stimulating activity from L. edodes contained mainly neutral sugars (58.7%) with considerable amounts of uronic acid (32.2%) and a small amount of proteins (9.1%). Component sugars of exo-biopolymer consisted of mainly arabinose, galactose, glucose, mannose, and xylose (0.95:0.81:0.96:1.00: 0.39, respectively). When the exo-biopolymer was treated with NaIO4, NaClO2, and pronase, the NaClO2 treatment and pronase digestion had little effect, whereas NaIO4 oxidation significantly decreased the macrophage stimulating activity (47.6% reduction at 100 μg/ml). Therefore, the carbohydrate moiety in exo-biopolymer from L. edodes plays an important role in the expression of the macrophage stimulating activity.

AB - To find a new utilization of rice bran, nine higher fungi were examined for the production of exo-biopolymer with macrophage stimulating activity from rice bran. Among the exo-biopolymers produced from submerged cultures, Lentinus edodes showed the highest activity, followed by Grifola frondosa, Schizophyllum commune, and Coriolus versicolor. L. edodes also had the most potent macrophage stimulating activity in a liquid culture rather than in a solid culture. In order to improve rice bran utilization and the yield of exo-biopolymer with macrophage stimulating activity, the treatment of Rapidase effectively increased the macrophage stimulating activity (about 30% increase), whereas the other enzymes (Econase, Viscozyme, Ultraflo, Celluclast, and Thermylase) treatments did not increase the macrophage stimulating activity. Exo-biopolymer with macrophage stimulating activity from L. edodes contained mainly neutral sugars (58.7%) with considerable amounts of uronic acid (32.2%) and a small amount of proteins (9.1%). Component sugars of exo-biopolymer consisted of mainly arabinose, galactose, glucose, mannose, and xylose (0.95:0.81:0.96:1.00: 0.39, respectively). When the exo-biopolymer was treated with NaIO4, NaClO2, and pronase, the NaClO2 treatment and pronase digestion had little effect, whereas NaIO4 oxidation significantly decreased the macrophage stimulating activity (47.6% reduction at 100 μg/ml). Therefore, the carbohydrate moiety in exo-biopolymer from L. edodes plays an important role in the expression of the macrophage stimulating activity.

KW - Exo-biopolymer

KW - Lentinus edodes

KW - Macrophage stimulating activity

KW - Submerged culture

UR - http://www.scopus.com/inward/record.url?scp=4444245929&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4444245929&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:4444245929

VL - 14

SP - 658

EP - 664

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

SN - 1017-7825

IS - 4

ER -