Macrophageal/microglial cell activation and cerebral injury induced by excretory-secretory products secreted by Paragonimus westermani

Jae Chul Lee, Geum Sil Cho, Jae Hyun Kwon, Myeong Heon Shin, Ji Hyae Lim, Won-Ki Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Cerebral paragonimiasis causes various neurological disorders including seizures, visual impairment and hemiplegia. The excretory-secretory product (ESP) released by Paragonimus westermani has a cysteine protease activity and plays important roles in its migration in the host tissue and modulation of host immune responses. To gain more insight into the pathogenesis of ESP in the brain, we investigated the inflammatory reaction and cerebral injury following microinjection of ESP into rat striatum. The size of injury was maximally observed 3 days after microinjection of ESP and then declined to control levels as astrocytes have repopulated the injury. ED1-positive monocytes and microglia were confluently found inside the injury. The mRNA expression of inducible nitric oxide synthase (iNOS) occurred as early as 9 h after ESP injection and then declined to control levels within 1 day. The iNOS inhibitor aminoguanidine largely decreased the expression of iNOS but did not reduce the size of lesion caused by ESP. Interestingly, however, heat inactivation of ESP caused a decrease of injury formation with no altered expression of iNOS. The data indicate that ESP produces brain tissue injury by recruiting activated monocytes/microglia via heat-labile protease activity.

Original languageEnglish
Pages (from-to)133-139
Number of pages7
JournalNeuroscience Research
Volume54
Issue number2
DOIs
Publication statusPublished - 2006 Feb 1
Externally publishedYes

Keywords

  • Astrocyte
  • Excretory-secretory product (ESP)
  • iNOS
  • Microglia
  • Monocyte

ASJC Scopus subject areas

  • Neuroscience(all)

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