Magnesium ion selective two-photon fluorescent probe based on a benzo[h]chromene derivative for in vivo imaging

Hwan Myung Kim, Pil Rye Yang, Mun Sik Seo, Jae Sung Yi, Jin Hee Hong, Seung-Joon Jeon, Young-Gyu Ko, Kyoung Jin Lee, Bong-Rae Cho

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

A novel, two-photon probe for the detection of free Mg2+ ions in living cells and live tissues has been developed. The probe can be excited by 880 nm laser photons, emits strong two-photon excited fluorescence in response to Mg2+ ions, can be easily loaded into the cell and tissue, shows high photostability, and can measure the Mg2+ ion concentration without interference by Ca2+ ions in living cells. The intracellular dissociation constant (Kdi) for Mg2+ determined by the two-photon process is 2.5 mM, which is suitable for dynamic Mg 2+ concentration measurement. In addition, the probe is capable of imaging endogenous stores of free Mg2+ at a few hundred micrometers depth in live tissues using two-photon microscopy (TPM).

Original languageEnglish
Pages (from-to)2088-2096
Number of pages9
JournalJournal of Organic Chemistry
Volume72
Issue number6
DOIs
Publication statusPublished - 2007 Mar 16

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Benzopyrans
Fluorescent Dyes
Photons
Magnesium
Ions
Derivatives
Imaging techniques
Tissue
Two photon processes
Cells
Microscopic examination
Fluorescence
Microscopy
Lasers

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Magnesium ion selective two-photon fluorescent probe based on a benzo[h]chromene derivative for in vivo imaging. / Kim, Hwan Myung; Yang, Pil Rye; Seo, Mun Sik; Yi, Jae Sung; Hong, Jin Hee; Jeon, Seung-Joon; Ko, Young-Gyu; Lee, Kyoung Jin; Cho, Bong-Rae.

In: Journal of Organic Chemistry, Vol. 72, No. 6, 16.03.2007, p. 2088-2096.

Research output: Contribution to journalArticle

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AU - Hong, Jin Hee

AU - Jeon, Seung-Joon

AU - Ko, Young-Gyu

AU - Lee, Kyoung Jin

AU - Cho, Bong-Rae

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