Magnesium ion selective two-photon fluorescent probe based on a benzo[h]chromene derivative for in vivo imaging

Hwan Myung Kim, Pil Rye Yang, Mun Sik Seo, Jae Sung Yi, Jin Hee Hong, Seung Joon Jeon, Young Gyu Ko, Kyoung J. Lee, Bong Rae Cho

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

A novel, two-photon probe for the detection of free Mg2+ ions in living cells and live tissues has been developed. The probe can be excited by 880 nm laser photons, emits strong two-photon excited fluorescence in response to Mg2+ ions, can be easily loaded into the cell and tissue, shows high photostability, and can measure the Mg2+ ion concentration without interference by Ca2+ ions in living cells. The intracellular dissociation constant (Kdi) for Mg2+ determined by the two-photon process is 2.5 mM, which is suitable for dynamic Mg 2+ concentration measurement. In addition, the probe is capable of imaging endogenous stores of free Mg2+ at a few hundred micrometers depth in live tissues using two-photon microscopy (TPM).

Original languageEnglish
Pages (from-to)2088-2096
Number of pages9
JournalJournal of Organic Chemistry
Volume72
Issue number6
DOIs
Publication statusPublished - 2007 Mar 16

ASJC Scopus subject areas

  • Organic Chemistry

Fingerprint Dive into the research topics of 'Magnesium ion selective two-photon fluorescent probe based on a benzo[h]chromene derivative for in vivo imaging'. Together they form a unique fingerprint.

  • Cite this