Magnetophoretic sorting of microdroplets with different microalgal cell densities for rapid isolation of fast growing strains

Young Joon Sung, Jaoon Young Hwan Kim, Hong Il Choi, Ho Seok Kwak, Sang Jun Sim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Microalgae-unicellular photosynthetic organisms-have received increasing attention for their ability to biologically convert CO2 into valuable products. The commercial use of microalgae requires screening strains to improve the biomass productivity to achieve a high-Throughput. Here, we developed a microfluidic method that uses a magnetic field to separate the microdroplets containing different concentrations of microalgal cells. The separation efficiency is maximized using the following parameters that influence the amount of lateral displacement of the microdroplets: magnetic nanoparticle concentration, flow rate of droplets, x-and y-Axis location of the magnet, and diameter of the droplets. Consequently, 91.90% of empty, 87.12% of low-, and 90.66% of high-density droplets could be separated into different outlets through simple manipulation of the magnetic field in the microfluidic device. These results indicate that cell density-based separation of microdroplets using a magnetic force can provide a promising platform to isolate microalgal species with a high growth performance.

Original languageEnglish
Article number10390
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

Fingerprint

Sorting
Microfluidics
Magnetic fields
Magnets
Screening
Biomass
Productivity
Cells
Flow rate
Throughput
Nanoparticles

ASJC Scopus subject areas

  • General

Cite this

Magnetophoretic sorting of microdroplets with different microalgal cell densities for rapid isolation of fast growing strains. / Sung, Young Joon; Kim, Jaoon Young Hwan; Choi, Hong Il; Kwak, Ho Seok; Sim, Sang Jun.

In: Scientific Reports, Vol. 7, No. 1, 10390, 01.12.2017.

Research output: Contribution to journalArticle

@article{9f4105a538b142ccb5dac3c674612477,
title = "Magnetophoretic sorting of microdroplets with different microalgal cell densities for rapid isolation of fast growing strains",
abstract = "Microalgae-unicellular photosynthetic organisms-have received increasing attention for their ability to biologically convert CO2 into valuable products. The commercial use of microalgae requires screening strains to improve the biomass productivity to achieve a high-Throughput. Here, we developed a microfluidic method that uses a magnetic field to separate the microdroplets containing different concentrations of microalgal cells. The separation efficiency is maximized using the following parameters that influence the amount of lateral displacement of the microdroplets: magnetic nanoparticle concentration, flow rate of droplets, x-and y-Axis location of the magnet, and diameter of the droplets. Consequently, 91.90{\%} of empty, 87.12{\%} of low-, and 90.66{\%} of high-density droplets could be separated into different outlets through simple manipulation of the magnetic field in the microfluidic device. These results indicate that cell density-based separation of microdroplets using a magnetic force can provide a promising platform to isolate microalgal species with a high growth performance.",
author = "Sung, {Young Joon} and Kim, {Jaoon Young Hwan} and Choi, {Hong Il} and Kwak, {Ho Seok} and Sim, {Sang Jun}",
year = "2017",
month = "12",
day = "1",
doi = "10.1038/s41598-017-10764-6",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Magnetophoretic sorting of microdroplets with different microalgal cell densities for rapid isolation of fast growing strains

AU - Sung, Young Joon

AU - Kim, Jaoon Young Hwan

AU - Choi, Hong Il

AU - Kwak, Ho Seok

AU - Sim, Sang Jun

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Microalgae-unicellular photosynthetic organisms-have received increasing attention for their ability to biologically convert CO2 into valuable products. The commercial use of microalgae requires screening strains to improve the biomass productivity to achieve a high-Throughput. Here, we developed a microfluidic method that uses a magnetic field to separate the microdroplets containing different concentrations of microalgal cells. The separation efficiency is maximized using the following parameters that influence the amount of lateral displacement of the microdroplets: magnetic nanoparticle concentration, flow rate of droplets, x-and y-Axis location of the magnet, and diameter of the droplets. Consequently, 91.90% of empty, 87.12% of low-, and 90.66% of high-density droplets could be separated into different outlets through simple manipulation of the magnetic field in the microfluidic device. These results indicate that cell density-based separation of microdroplets using a magnetic force can provide a promising platform to isolate microalgal species with a high growth performance.

AB - Microalgae-unicellular photosynthetic organisms-have received increasing attention for their ability to biologically convert CO2 into valuable products. The commercial use of microalgae requires screening strains to improve the biomass productivity to achieve a high-Throughput. Here, we developed a microfluidic method that uses a magnetic field to separate the microdroplets containing different concentrations of microalgal cells. The separation efficiency is maximized using the following parameters that influence the amount of lateral displacement of the microdroplets: magnetic nanoparticle concentration, flow rate of droplets, x-and y-Axis location of the magnet, and diameter of the droplets. Consequently, 91.90% of empty, 87.12% of low-, and 90.66% of high-density droplets could be separated into different outlets through simple manipulation of the magnetic field in the microfluidic device. These results indicate that cell density-based separation of microdroplets using a magnetic force can provide a promising platform to isolate microalgal species with a high growth performance.

UR - http://www.scopus.com/inward/record.url?scp=85028768155&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85028768155&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-10764-6

DO - 10.1038/s41598-017-10764-6

M3 - Article

C2 - 28871196

AN - SCOPUS:85028768155

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 10390

ER -