TY - JOUR
T1 - Maldi-tof analysis of polyhexamethylene guanidine (phmg) oligomers used as a commercial antibacterial humidifier disinfectant
AU - Hwang, Hyo Jin
AU - Nam, Jungjoo
AU - Yang, Sung Ik
AU - Kwon, Jung Hwan
AU - Oh, Han Bin
PY - 2013/6/20
Y1 - 2013/6/20
N2 - Polyhexamethylene guanidine (PHMG) polymers used as an active ingredient in an antibacterial humidifier disinfectant were reported to cause harm to the human health when inhaled, although physical contact with this material was known to present low toxicity to humans. It is therefore necessary to develop an optimal analysis method which enables detection and analysis of PHMG polymers. MALDI-TOF investigations of PHMG are performed with a variety of matrices, and it is found that CHCA and 2,5-DHB are excellent matrices which well reflects the polymer population even at high mass. For the provided PHMG sample, the number-average (Mn) and weight-average (Mw) molecular masses were determined to be 744.8 and 810.7, respectively, when the CHCA was used as a matrix. The rank of the matrices in terms of averaged molecular weight was CHCA ∼2,5-DHB > 5-NSA > DHAP, THAP > ATT > IAA ∼ super-DHB ∼ HABA. In addition, PSD of the PHMG oligomer ions exhibited a few unique fragmenation characteristics. The formation of a-and c-type fragments was the major fragmentation pathway, and the 25-Da loss peaks generally accompanied a-and c-type fragments.
AB - Polyhexamethylene guanidine (PHMG) polymers used as an active ingredient in an antibacterial humidifier disinfectant were reported to cause harm to the human health when inhaled, although physical contact with this material was known to present low toxicity to humans. It is therefore necessary to develop an optimal analysis method which enables detection and analysis of PHMG polymers. MALDI-TOF investigations of PHMG are performed with a variety of matrices, and it is found that CHCA and 2,5-DHB are excellent matrices which well reflects the polymer population even at high mass. For the provided PHMG sample, the number-average (Mn) and weight-average (Mw) molecular masses were determined to be 744.8 and 810.7, respectively, when the CHCA was used as a matrix. The rank of the matrices in terms of averaged molecular weight was CHCA ∼2,5-DHB > 5-NSA > DHAP, THAP > ATT > IAA ∼ super-DHB ∼ HABA. In addition, PSD of the PHMG oligomer ions exhibited a few unique fragmenation characteristics. The formation of a-and c-type fragments was the major fragmentation pathway, and the 25-Da loss peaks generally accompanied a-and c-type fragments.
KW - MALDI-TOF
KW - Mass spectrometry
KW - Matrix
KW - Polyhexamethylene guanidine (PHMG)
KW - Polymer
UR - http://www.scopus.com/inward/record.url?scp=84879203925&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84879203925&partnerID=8YFLogxK
U2 - 10.5012/bkcs.2013.34.6.1708
DO - 10.5012/bkcs.2013.34.6.1708
M3 - Article
AN - SCOPUS:84879203925
VL - 34
SP - 1708
EP - 1714
JO - Bulletin of the Korean Chemical Society
JF - Bulletin of the Korean Chemical Society
SN - 0253-2964
IS - 6
ER -