Maltosylated polyethylenimine-based triple nanocomplexes of human papillomavirus 16L1 protein and DNA as a vaccine co-delivery system

Hee Jeong Cho, Su Eun Han, Saewon Im, Youngsik Lee, Young Bong Kim, Taehoon Chun, Yu Kyoung Oh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To improve vaccine delivery, we herein designed a co-delivery system using a protein antigen and its encoding plasmid linked in nanocomplexes via maltosylated PEI (mPEI). Cationic mPEI was electrostatically complexed to a plasmid encoding the human papillomavirus (HPV) type 16L1 protein (pHPV16L1), and further complexed to a maltose binding protein (MBP)-fused human papillomavirus type 16L1 fusion protein (HPV16L1-MBP). The HPV16L1-MBP/mPEI/pHPV16L1 complexes were characterized by gel-retardation properties, zeta potentials and sizes. The intracellular co-delivery of protein and plasmid DNA vaccines was significantly higher for mPEI-based triple nanocomplexes than for a simple physical mixture of the proteins and DNA. Moreover, the cellular delivery of plasmid DNA using mPEI-based triple nanocomplexes resulted in higher expression levels comparable to those obtained using dual complexes of mPEI and the plasmid DNA. In vivo, co-immunization of mice with HPV16L1-MBP/mPEI/pHPV16L1 nanocomplexes triggered the highest levels of humoral immune responses among various vaccination groups. Moreover, the mPEI-based nanocomplexes significantly enhanced the number of interferon-γ producing CD8+ T cells compared with the use of mixed proteins and plasmid DNA. These results suggest that the effective cellular co-delivery of MBP-fused antigen proteins and plasmid DNA using maltosylated PEI-based triple nanocomplexes could enhance the immunogenicity of HPV16L1 vaccines.

Original languageEnglish
Pages (from-to)4621-4629
Number of pages9
JournalBiomaterials
Volume32
Issue number20
DOIs
Publication statusPublished - 2011 Jul 1

Fingerprint

Polyethyleneimine
Polyetherimides
Vaccines
Maltose-Binding Proteins
Plasmids
DNA
Maltose
Proteins
Antigens
DNA Vaccines
Interferons
Immunization
Humoral Immunity
T-cells
Zeta potential
Vaccination
Gels
T-Lymphocytes
Fusion reactions
Carrier Proteins

Keywords

  • Human papillomavirus
  • Maltose binding protein-fused protein
  • Maltosylated polyethylenimine
  • Triple nanocomplexes
  • Vaccine co-delivery systems

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Maltosylated polyethylenimine-based triple nanocomplexes of human papillomavirus 16L1 protein and DNA as a vaccine co-delivery system. / Cho, Hee Jeong; Han, Su Eun; Im, Saewon; Lee, Youngsik; Kim, Young Bong; Chun, Taehoon; Oh, Yu Kyoung.

In: Biomaterials, Vol. 32, No. 20, 01.07.2011, p. 4621-4629.

Research output: Contribution to journalArticle

Cho, Hee Jeong ; Han, Su Eun ; Im, Saewon ; Lee, Youngsik ; Kim, Young Bong ; Chun, Taehoon ; Oh, Yu Kyoung. / Maltosylated polyethylenimine-based triple nanocomplexes of human papillomavirus 16L1 protein and DNA as a vaccine co-delivery system. In: Biomaterials. 2011 ; Vol. 32, No. 20. pp. 4621-4629.
@article{8567fe61cc124de5b6004b4556b9b459,
title = "Maltosylated polyethylenimine-based triple nanocomplexes of human papillomavirus 16L1 protein and DNA as a vaccine co-delivery system",
abstract = "To improve vaccine delivery, we herein designed a co-delivery system using a protein antigen and its encoding plasmid linked in nanocomplexes via maltosylated PEI (mPEI). Cationic mPEI was electrostatically complexed to a plasmid encoding the human papillomavirus (HPV) type 16L1 protein (pHPV16L1), and further complexed to a maltose binding protein (MBP)-fused human papillomavirus type 16L1 fusion protein (HPV16L1-MBP). The HPV16L1-MBP/mPEI/pHPV16L1 complexes were characterized by gel-retardation properties, zeta potentials and sizes. The intracellular co-delivery of protein and plasmid DNA vaccines was significantly higher for mPEI-based triple nanocomplexes than for a simple physical mixture of the proteins and DNA. Moreover, the cellular delivery of plasmid DNA using mPEI-based triple nanocomplexes resulted in higher expression levels comparable to those obtained using dual complexes of mPEI and the plasmid DNA. In vivo, co-immunization of mice with HPV16L1-MBP/mPEI/pHPV16L1 nanocomplexes triggered the highest levels of humoral immune responses among various vaccination groups. Moreover, the mPEI-based nanocomplexes significantly enhanced the number of interferon-γ producing CD8+ T cells compared with the use of mixed proteins and plasmid DNA. These results suggest that the effective cellular co-delivery of MBP-fused antigen proteins and plasmid DNA using maltosylated PEI-based triple nanocomplexes could enhance the immunogenicity of HPV16L1 vaccines.",
keywords = "Human papillomavirus, Maltose binding protein-fused protein, Maltosylated polyethylenimine, Triple nanocomplexes, Vaccine co-delivery systems",
author = "Cho, {Hee Jeong} and Han, {Su Eun} and Saewon Im and Youngsik Lee and Kim, {Young Bong} and Taehoon Chun and Oh, {Yu Kyoung}",
year = "2011",
month = "7",
day = "1",
doi = "10.1016/j.biomaterials.2011.03.004",
language = "English",
volume = "32",
pages = "4621--4629",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "20",

}

TY - JOUR

T1 - Maltosylated polyethylenimine-based triple nanocomplexes of human papillomavirus 16L1 protein and DNA as a vaccine co-delivery system

AU - Cho, Hee Jeong

AU - Han, Su Eun

AU - Im, Saewon

AU - Lee, Youngsik

AU - Kim, Young Bong

AU - Chun, Taehoon

AU - Oh, Yu Kyoung

PY - 2011/7/1

Y1 - 2011/7/1

N2 - To improve vaccine delivery, we herein designed a co-delivery system using a protein antigen and its encoding plasmid linked in nanocomplexes via maltosylated PEI (mPEI). Cationic mPEI was electrostatically complexed to a plasmid encoding the human papillomavirus (HPV) type 16L1 protein (pHPV16L1), and further complexed to a maltose binding protein (MBP)-fused human papillomavirus type 16L1 fusion protein (HPV16L1-MBP). The HPV16L1-MBP/mPEI/pHPV16L1 complexes were characterized by gel-retardation properties, zeta potentials and sizes. The intracellular co-delivery of protein and plasmid DNA vaccines was significantly higher for mPEI-based triple nanocomplexes than for a simple physical mixture of the proteins and DNA. Moreover, the cellular delivery of plasmid DNA using mPEI-based triple nanocomplexes resulted in higher expression levels comparable to those obtained using dual complexes of mPEI and the plasmid DNA. In vivo, co-immunization of mice with HPV16L1-MBP/mPEI/pHPV16L1 nanocomplexes triggered the highest levels of humoral immune responses among various vaccination groups. Moreover, the mPEI-based nanocomplexes significantly enhanced the number of interferon-γ producing CD8+ T cells compared with the use of mixed proteins and plasmid DNA. These results suggest that the effective cellular co-delivery of MBP-fused antigen proteins and plasmid DNA using maltosylated PEI-based triple nanocomplexes could enhance the immunogenicity of HPV16L1 vaccines.

AB - To improve vaccine delivery, we herein designed a co-delivery system using a protein antigen and its encoding plasmid linked in nanocomplexes via maltosylated PEI (mPEI). Cationic mPEI was electrostatically complexed to a plasmid encoding the human papillomavirus (HPV) type 16L1 protein (pHPV16L1), and further complexed to a maltose binding protein (MBP)-fused human papillomavirus type 16L1 fusion protein (HPV16L1-MBP). The HPV16L1-MBP/mPEI/pHPV16L1 complexes were characterized by gel-retardation properties, zeta potentials and sizes. The intracellular co-delivery of protein and plasmid DNA vaccines was significantly higher for mPEI-based triple nanocomplexes than for a simple physical mixture of the proteins and DNA. Moreover, the cellular delivery of plasmid DNA using mPEI-based triple nanocomplexes resulted in higher expression levels comparable to those obtained using dual complexes of mPEI and the plasmid DNA. In vivo, co-immunization of mice with HPV16L1-MBP/mPEI/pHPV16L1 nanocomplexes triggered the highest levels of humoral immune responses among various vaccination groups. Moreover, the mPEI-based nanocomplexes significantly enhanced the number of interferon-γ producing CD8+ T cells compared with the use of mixed proteins and plasmid DNA. These results suggest that the effective cellular co-delivery of MBP-fused antigen proteins and plasmid DNA using maltosylated PEI-based triple nanocomplexes could enhance the immunogenicity of HPV16L1 vaccines.

KW - Human papillomavirus

KW - Maltose binding protein-fused protein

KW - Maltosylated polyethylenimine

KW - Triple nanocomplexes

KW - Vaccine co-delivery systems

UR - http://www.scopus.com/inward/record.url?scp=79955521051&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955521051&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2011.03.004

DO - 10.1016/j.biomaterials.2011.03.004

M3 - Article

C2 - 21440296

AN - SCOPUS:79955521051

VL - 32

SP - 4621

EP - 4629

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 20

ER -