Mammalian mitochondrial endonuclease activities specific for ultraviolet-irradiated DNA

Alan E. Tomkinson, R. Thomas Bonk, Joon Kim, Neil Bartfeld, Stuart Linn

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Mitochondrial forms of uracil DNA glycosylase and UV endonuclease have been purified and characterized from the mouse plasmacytoma cell line, MPC-11. As in other cell types, the mitochondrial uracil DNA glycosylase has properties very similar to those of the nuclear enzyme, although in this case the mitochondrial activity was also distinguishable by extreme sensitivity to dilution. Three mitochondrial UV endonuclease activities are also similar to nuclear enzymes; however, the relative amounts of these enzyme activities in the mitochondria is significantly different from that in the nucleus. In particular, mitochondria contain a much higher proportion of an activity analogous to UV endonuclease III. Nuclear UV endonuclease III activity is absent from XP group D fibroblasts and XP group D lymphoblasts have reduced, but detectable levels of the mitochondrial form of this enzyme. This residual activity differs in its properties from the normal mitochondrial form of UV endonuclease III, however. The presence of these enzyme activities which function in base excision repair suggests that such DNA repair occurs in mitochondria. Alternatively, these enzymes might act to mark damaged mitochondrial genomes for subsequent degradation.

Original languageEnglish
Pages (from-to)929-935
Number of pages7
JournalNucleic Acids Research
Volume18
Issue number4
Publication statusPublished - 1990 Feb 25
Externally publishedYes

Fingerprint

Endonucleases
Ultraviolet
DNA
Mitochondria
Enzymes
Uracil-DNA Glycosidase
Enzyme activity
Repair
DNA Repair
Fibroblasts
Plasmacytoma
Mitochondrial Genome
Deoxyribonuclease I
Dilution
Mitochondrial DNA
Cell
Genes
Cells
UV endonuclease
Normal Form

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Tomkinson, A. E., Bonk, R. T., Kim, J., Bartfeld, N., & Linn, S. (1990). Mammalian mitochondrial endonuclease activities specific for ultraviolet-irradiated DNA. Nucleic Acids Research, 18(4), 929-935.

Mammalian mitochondrial endonuclease activities specific for ultraviolet-irradiated DNA. / Tomkinson, Alan E.; Bonk, R. Thomas; Kim, Joon; Bartfeld, Neil; Linn, Stuart.

In: Nucleic Acids Research, Vol. 18, No. 4, 25.02.1990, p. 929-935.

Research output: Contribution to journalArticle

Tomkinson, AE, Bonk, RT, Kim, J, Bartfeld, N & Linn, S 1990, 'Mammalian mitochondrial endonuclease activities specific for ultraviolet-irradiated DNA', Nucleic Acids Research, vol. 18, no. 4, pp. 929-935.
Tomkinson, Alan E. ; Bonk, R. Thomas ; Kim, Joon ; Bartfeld, Neil ; Linn, Stuart. / Mammalian mitochondrial endonuclease activities specific for ultraviolet-irradiated DNA. In: Nucleic Acids Research. 1990 ; Vol. 18, No. 4. pp. 929-935.
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