Mammals reduce methionine-S-sulfoxide with MsrA and are unable to reduce methionine-R-sulfoxide, and this function can be restored with a yeast reductase

Byung Cheon Lee, Tien Le Dung, Vadim N. Gladyshev

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Methionine is an essential amino acid in mammals at the junction of methylation, protein synthesis, and sulfur pathways. However, this amino acid is highly susceptible to oxidation, resulting in a mixture of methionine-S- sulfoxide and methionine-R-sulfoxide. Whether methionine is quantitatively regenerated from these compounds is unknown. Here we report that SK-Hep1 hepatocytes grew on methionine-S-sulfoxide and consumed this compound by import and methionine-S-sulfoxide reductase (MsrA)-dependent reduction, but methionine-R-sulfoxide reductases were not involved in this process, and methionine-R-sulfoxide could not be used by the cells. However, SK-Hep1 cells expressing a yeast free methionine-R-sulfoxide reductase proliferated in the presence of either sulfoxide, reduced them, and showed increased resistance to oxidative stress. Only methionine-R-sulfoxide was detected in the plasma of wild type mice, but both sulfoxides were in the plasma of MsrA knock-out mice. These results show thatmammalscan support methionine metabolism by reduction of methionine-S-sulfoxide, that this process is dependent on MsrA, that mammals are inherently deficient in the reduction of methionine-R-sulfoxide, and that expression of yeast free methionine-R-sulfoxide reductase can fully compensate for this deficiency.

Original languageEnglish
Pages (from-to)28361-28369
Number of pages9
JournalJournal of Biological Chemistry
Volume283
Issue number42
DOIs
Publication statusPublished - 2008 Oct 17
Externally publishedYes

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sulfoxide
Mammals
Methionine
Yeast
Oxidoreductases
Yeasts
Methionine Sulfoxide Reductases
Sulfoxides
Essential Amino Acids
sulfoxide reductase
methionine sulfoxide
methionine sulfoxide reductase
Sulfur
Knockout Mice
Methylation
Hepatocytes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Mammals reduce methionine-S-sulfoxide with MsrA and are unable to reduce methionine-R-sulfoxide, and this function can be restored with a yeast reductase. / Lee, Byung Cheon; Dung, Tien Le; Gladyshev, Vadim N.

In: Journal of Biological Chemistry, Vol. 283, No. 42, 17.10.2008, p. 28361-28369.

Research output: Contribution to journalArticle

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