Manipulation of cells using an ultrasonic pressure field

Albrecht Haake, Adrian Neild, Deok Ho Kim, Jong Eun Ihm, Yu Sun, Jürg Dual, Byeong Kwon Ju

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

A novel device is described that generates an ultrasonic force field in a fluid layer. The force field arises because of the acoustic radiation force, a second order effect, generated as an ultrasonic wave interacts with a suspended particle. This force field can be used to manipulate objects in the fluid layer trapped between this device and an arbitrary surface, in this case, a flat object slide. The device is shown to be capable of positioning and, in doing so, concentrating human cells to predictable locations. Mesenchymal and HeLa cells were used. Critically, the forces required to do this can be generated by ultrasonic pressure fields that do not affect the viability of the cells. The viability has been assessed using trypan blue dye. The device used consists of a 14 mm square glass plate that is excited by at least one of four piezotransducers attached to the edges. The resulting ultrasonic force field and, importantly, the location of the minima in the force potential at which the cells are collected, has been calculated analytically.

Original languageEnglish
Pages (from-to)857-864
Number of pages8
JournalUltrasound in Medicine and Biology
Volume31
Issue number6
DOIs
Publication statusPublished - 2005 Jun 1
Externally publishedYes

Fingerprint

Ultrasonics
Pressure
Equipment and Supplies
Trypan Blue
HeLa Cells
Acoustics
Glass
Cell Survival
Coloring Agents
Radiation

Keywords

  • Lamb wave
  • Manipulation
  • Positioning
  • Standing wave

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Manipulation of cells using an ultrasonic pressure field. / Haake, Albrecht; Neild, Adrian; Kim, Deok Ho; Ihm, Jong Eun; Sun, Yu; Dual, Jürg; Ju, Byeong Kwon.

In: Ultrasound in Medicine and Biology, Vol. 31, No. 6, 01.06.2005, p. 857-864.

Research output: Contribution to journalArticle

Haake, Albrecht ; Neild, Adrian ; Kim, Deok Ho ; Ihm, Jong Eun ; Sun, Yu ; Dual, Jürg ; Ju, Byeong Kwon. / Manipulation of cells using an ultrasonic pressure field. In: Ultrasound in Medicine and Biology. 2005 ; Vol. 31, No. 6. pp. 857-864.
@article{1a923a27bdcc476181fbdeb3fabc7b2b,
title = "Manipulation of cells using an ultrasonic pressure field",
abstract = "A novel device is described that generates an ultrasonic force field in a fluid layer. The force field arises because of the acoustic radiation force, a second order effect, generated as an ultrasonic wave interacts with a suspended particle. This force field can be used to manipulate objects in the fluid layer trapped between this device and an arbitrary surface, in this case, a flat object slide. The device is shown to be capable of positioning and, in doing so, concentrating human cells to predictable locations. Mesenchymal and HeLa cells were used. Critically, the forces required to do this can be generated by ultrasonic pressure fields that do not affect the viability of the cells. The viability has been assessed using trypan blue dye. The device used consists of a 14 mm square glass plate that is excited by at least one of four piezotransducers attached to the edges. The resulting ultrasonic force field and, importantly, the location of the minima in the force potential at which the cells are collected, has been calculated analytically.",
keywords = "Lamb wave, Manipulation, Positioning, Standing wave",
author = "Albrecht Haake and Adrian Neild and Kim, {Deok Ho} and Ihm, {Jong Eun} and Yu Sun and J{\"u}rg Dual and Ju, {Byeong Kwon}",
year = "2005",
month = "6",
day = "1",
doi = "10.1016/j.ultrasmedbio.2005.03.004",
language = "English",
volume = "31",
pages = "857--864",
journal = "Ultrasound in Medicine and Biology",
issn = "0301-5629",
publisher = "Elsevier USA",
number = "6",

}

TY - JOUR

T1 - Manipulation of cells using an ultrasonic pressure field

AU - Haake, Albrecht

AU - Neild, Adrian

AU - Kim, Deok Ho

AU - Ihm, Jong Eun

AU - Sun, Yu

AU - Dual, Jürg

AU - Ju, Byeong Kwon

PY - 2005/6/1

Y1 - 2005/6/1

N2 - A novel device is described that generates an ultrasonic force field in a fluid layer. The force field arises because of the acoustic radiation force, a second order effect, generated as an ultrasonic wave interacts with a suspended particle. This force field can be used to manipulate objects in the fluid layer trapped between this device and an arbitrary surface, in this case, a flat object slide. The device is shown to be capable of positioning and, in doing so, concentrating human cells to predictable locations. Mesenchymal and HeLa cells were used. Critically, the forces required to do this can be generated by ultrasonic pressure fields that do not affect the viability of the cells. The viability has been assessed using trypan blue dye. The device used consists of a 14 mm square glass plate that is excited by at least one of four piezotransducers attached to the edges. The resulting ultrasonic force field and, importantly, the location of the minima in the force potential at which the cells are collected, has been calculated analytically.

AB - A novel device is described that generates an ultrasonic force field in a fluid layer. The force field arises because of the acoustic radiation force, a second order effect, generated as an ultrasonic wave interacts with a suspended particle. This force field can be used to manipulate objects in the fluid layer trapped between this device and an arbitrary surface, in this case, a flat object slide. The device is shown to be capable of positioning and, in doing so, concentrating human cells to predictable locations. Mesenchymal and HeLa cells were used. Critically, the forces required to do this can be generated by ultrasonic pressure fields that do not affect the viability of the cells. The viability has been assessed using trypan blue dye. The device used consists of a 14 mm square glass plate that is excited by at least one of four piezotransducers attached to the edges. The resulting ultrasonic force field and, importantly, the location of the minima in the force potential at which the cells are collected, has been calculated analytically.

KW - Lamb wave

KW - Manipulation

KW - Positioning

KW - Standing wave

UR - http://www.scopus.com/inward/record.url?scp=20144384756&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20144384756&partnerID=8YFLogxK

U2 - 10.1016/j.ultrasmedbio.2005.03.004

DO - 10.1016/j.ultrasmedbio.2005.03.004

M3 - Article

VL - 31

SP - 857

EP - 864

JO - Ultrasound in Medicine and Biology

JF - Ultrasound in Medicine and Biology

SN - 0301-5629

IS - 6

ER -