Manipulation of the genes for poly-β-hydroxybutyric acid synthesis in Alcaligenes eutrophus

Hae Chul Park, Jin Seo Park, Yong Hyun Lee, Tae Lin Huh

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In order to establish the molecular breeding system in Alcaligenes eutrophus producing poly-β-hydroxybutyric acid (PHB), phbCAB genes from A. eutrophus were recombined into the E. coli-A. eutrophus shuttle vector and directly transferred into A. eutrophus by the electroporation. In A. eutrophus transformants, recombinant plasmids were stably maintained and enzyme activities for PHB biosyntheses were elevated 1.4-2.7 fold by the cloned genes.

Original languageEnglish
Pages (from-to)729-734
Number of pages6
JournalBiotechnology letters
Issue number7
Publication statusPublished - 1995 Jul 1


ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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