Manipulation of the genes for poly-β-hydroxybutyric acid synthesis in Alcaligenes eutrophus

Hae Chul Park, J. S. Park, Y. H. Lee, T. L. Huh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In order to establish the molecular breeding system in Alcaligenes eutrophus producing poly-β-hydroxybutyric acid (PHB), phbCAB genes from A. eletrophus were recombined into the E. coli-A. eutrophus shuttle vector and directly transferred into A. eutrophus by the electroporation. In A. eutrophus transformants, recombinant plasmids were stably maintained and enzyme activities for PHB biosyntheses were elevated 1.4-2.7 fold by the cloned genes.

Original languageEnglish
Pages (from-to)729-734
Number of pages6
JournalBiotechnology Letters
Volume17
Issue number7
DOIs
Publication statusPublished - 1995 Aug 22
Externally publishedYes

Fingerprint

Hydroxybutyrates
Alcaligenes
Genes
DNA Shuffling
Genetic Vectors
Electroporation
Acids
Biosynthesis
Enzyme activity
Escherichia coli
Plasmids
Enzymes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Manipulation of the genes for poly-β-hydroxybutyric acid synthesis in Alcaligenes eutrophus. / Park, Hae Chul; Park, J. S.; Lee, Y. H.; Huh, T. L.

In: Biotechnology Letters, Vol. 17, No. 7, 22.08.1995, p. 729-734.

Research output: Contribution to journalArticle

Park, Hae Chul ; Park, J. S. ; Lee, Y. H. ; Huh, T. L. / Manipulation of the genes for poly-β-hydroxybutyric acid synthesis in Alcaligenes eutrophus. In: Biotechnology Letters. 1995 ; Vol. 17, No. 7. pp. 729-734.
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