Manufacture of elastic biodegradable PLCL scaffolds for mechano-active vascular tissue engineering

Sung In Jeong, Soo Hyun Kim, Young Ha Kim, Youngmee Jung, Jae Hyun Kwon, Byung Soo Kim, Young Moo Lee

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

A soft and very elastic poly(lactide-co-E-caprolactone) (PLCL)(50:50, Mn 185 × 103) was synthesized. Tubular scaffolds were prepared by an extrusion-particulate leaching method for mechano-active vascular tissue engineering. The copolymer was very flexible but completely rubber-like elastic. Even the high porous PLCL scaffolds (90% salt wt) exhibited 200% elongation, but recovery over 85% in a tensile test. Moreover, the PLCL scaffolds maintained their high elasticity also in culture media under cyclic mechanical strain conditions. The highly porous scaffold (90% salt wt) withstood for an initial 1 week without any deformation and sustained for 2 weeks in culture media under cyclic stress of 10% amplitude and at 1 Hz frequency which are similar to the natural vascular conditions. Vascular smooth muscle cells (VSMCs) were seeded on to the PLCL scaffolds. The cell adhesion and proliferation on the scaffolds of various pore-size were increased with increasing pore size. For the pore sizes of 50-100 μm, 100-150 μm, 150-200 μm and 200-250 μm, the ratios of cell numbers were about 1:1.2:1.9:2.2, respectively, at both 12 h and 5 days. Similarly, the higher porous scaffolds exhibited more cell adhesion and proliferation compared to lower porous one, where the effect was more pronounced in the longer proliferation period. SMC-seeded scaffolds were implanted subcutaneously in athymic nude mice to confirm the biocompatibility. Such a high elastic property and proper biocompatibility to SMCs of PLCL scaffolds prepared in this study will be very useful to engineer SM-containing tissues such as blood vessels under mechanically dynamic environments (mechano-active tissue engineering).

Original languageEnglish
Pages (from-to)645-660
Number of pages16
JournalJournal of Biomaterials Science, Polymer Edition
Volume15
Issue number5
DOIs
Publication statusPublished - 2004 Jul 12
Externally publishedYes

Fingerprint

Tissue Engineering
Scaffolds (biology)
Tissue engineering
Scaffolds
Blood Vessels
Nude Mice
Cell Adhesion
Culture Media
Pore size
Salts
Cell Proliferation
Cell adhesion
Cell proliferation
Biocompatibility
Rubber
Elasticity
Vascular Smooth Muscle
Smooth Muscle Myocytes
Cell Count
Blood vessels

Keywords

  • Elastic PLCL
  • Elastic scaffolds
  • Mechano-active tissue engineering
  • Smooth muscle cell

ASJC Scopus subject areas

  • Biophysics

Cite this

Manufacture of elastic biodegradable PLCL scaffolds for mechano-active vascular tissue engineering. / Jeong, Sung In; Kim, Soo Hyun; Kim, Young Ha; Jung, Youngmee; Kwon, Jae Hyun; Kim, Byung Soo; Lee, Young Moo.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 15, No. 5, 12.07.2004, p. 645-660.

Research output: Contribution to journalArticle

Jeong, Sung In ; Kim, Soo Hyun ; Kim, Young Ha ; Jung, Youngmee ; Kwon, Jae Hyun ; Kim, Byung Soo ; Lee, Young Moo. / Manufacture of elastic biodegradable PLCL scaffolds for mechano-active vascular tissue engineering. In: Journal of Biomaterials Science, Polymer Edition. 2004 ; Vol. 15, No. 5. pp. 645-660.
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