Abstract
The RNA promoters of the genome and antigenome of Newcastle disease virus (NDV) were studied by mutational analysis of their 3′ terminal ends. Similarly to other paramyxoviruses, NDV RNA replication follows the rule of six, and the genomic and antigenomic promoters require two discontinuous regions: conserved region I (first 18 nucleotides) and conserved region II (nucleotides 73-90). Proper spacing between those regions and the phase of six in region II is critical for efficient RNA promoter activity. As expected, the gene start signal at the 3′ end of the NDV genome was required for mRNA transcription, but not for RNA replication. Surprisingly, mutation of the polyadenylation signal in the 5′ end did not affect gene expression transcription. Although the conserved region I of NDV (avulavirus) promoter appears to be more similar to that of Sendai virus (SeV) (respirovirus), conserved region II is analogous to that of rubulaviruses.
| Original language | English |
|---|---|
| Pages (from-to) | 396-406 |
| Number of pages | 11 |
| Journal | Virology |
| Volume | 331 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2005 Jan 20 |
| Externally published | Yes |
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Keywords
- Newcastle disease virus
- Paramyxovirus
- RNA promoter
- RNA replication
- RNA transcription
ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Mapping of the RNA promoter of Newcastle disease virus. / Marcos, Fernando; Ferreira, Laura; Cros, Jerome; Park, Man-Seong; Nakaya, Takaaki; García-Sastre, Adolfo; Villar, Enrique.
In: Virology, Vol. 331, No. 2, 20.01.2005, p. 396-406.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mapping of the RNA promoter of Newcastle disease virus
AU - Marcos, Fernando
AU - Ferreira, Laura
AU - Cros, Jerome
AU - Park, Man-Seong
AU - Nakaya, Takaaki
AU - García-Sastre, Adolfo
AU - Villar, Enrique
PY - 2005/1/20
Y1 - 2005/1/20
N2 - The RNA promoters of the genome and antigenome of Newcastle disease virus (NDV) were studied by mutational analysis of their 3′ terminal ends. Similarly to other paramyxoviruses, NDV RNA replication follows the rule of six, and the genomic and antigenomic promoters require two discontinuous regions: conserved region I (first 18 nucleotides) and conserved region II (nucleotides 73-90). Proper spacing between those regions and the phase of six in region II is critical for efficient RNA promoter activity. As expected, the gene start signal at the 3′ end of the NDV genome was required for mRNA transcription, but not for RNA replication. Surprisingly, mutation of the polyadenylation signal in the 5′ end did not affect gene expression transcription. Although the conserved region I of NDV (avulavirus) promoter appears to be more similar to that of Sendai virus (SeV) (respirovirus), conserved region II is analogous to that of rubulaviruses.
AB - The RNA promoters of the genome and antigenome of Newcastle disease virus (NDV) were studied by mutational analysis of their 3′ terminal ends. Similarly to other paramyxoviruses, NDV RNA replication follows the rule of six, and the genomic and antigenomic promoters require two discontinuous regions: conserved region I (first 18 nucleotides) and conserved region II (nucleotides 73-90). Proper spacing between those regions and the phase of six in region II is critical for efficient RNA promoter activity. As expected, the gene start signal at the 3′ end of the NDV genome was required for mRNA transcription, but not for RNA replication. Surprisingly, mutation of the polyadenylation signal in the 5′ end did not affect gene expression transcription. Although the conserved region I of NDV (avulavirus) promoter appears to be more similar to that of Sendai virus (SeV) (respirovirus), conserved region II is analogous to that of rubulaviruses.
KW - Newcastle disease virus
KW - Paramyxovirus
KW - RNA promoter
KW - RNA replication
KW - RNA transcription
UR - http://www.scopus.com/inward/record.url?scp=11344256048&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=11344256048&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2004.10.040
DO - 10.1016/j.virol.2004.10.040
M3 - Article
C2 - 15629782
AN - SCOPUS:11344256048
VL - 331
SP - 396
EP - 406
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -