Mapping of the RNA promoter of Newcastle disease virus

Fernando Marcos; Laura Ferreira; Jerome Cros; Man Seong Park; Takaaki Nakaya; Adolfo García-Sastre; Enrique Villar

doi:10.1016/j.virol.2004.10.040

Mapping of the RNA promoter of Newcastle disease virus

Fernando Marcos, Laura Ferreira, Jerome Cros, Man Seong Park, Takaaki Nakaya, Adolfo García-Sastre, Enrique Villar

Department of Microbiology

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

The RNA promoters of the genome and antigenome of Newcastle disease virus (NDV) were studied by mutational analysis of their 3′ terminal ends. Similarly to other paramyxoviruses, NDV RNA replication follows the rule of six, and the genomic and antigenomic promoters require two discontinuous regions: conserved region I (first 18 nucleotides) and conserved region II (nucleotides 73-90). Proper spacing between those regions and the phase of six in region II is critical for efficient RNA promoter activity. As expected, the gene start signal at the 3′ end of the NDV genome was required for mRNA transcription, but not for RNA replication. Surprisingly, mutation of the polyadenylation signal in the 5′ end did not affect gene expression transcription. Although the conserved region I of NDV (avulavirus) promoter appears to be more similar to that of Sendai virus (SeV) (respirovirus), conserved region II is analogous to that of rubulaviruses.

Original language	English
Pages (from-to)	396-406
Number of pages	11
Journal	Virology
Volume	331
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2004.10.040
Publication status	Published - 2005 Jan 20

Keywords

Newcastle disease virus
Paramyxovirus
RNA promoter
RNA replication
RNA transcription

ASJC Scopus subject areas

Virology

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title = "Mapping of the RNA promoter of Newcastle disease virus",

abstract = "The RNA promoters of the genome and antigenome of Newcastle disease virus (NDV) were studied by mutational analysis of their 3′ terminal ends. Similarly to other paramyxoviruses, NDV RNA replication follows the rule of six, and the genomic and antigenomic promoters require two discontinuous regions: conserved region I (first 18 nucleotides) and conserved region II (nucleotides 73-90). Proper spacing between those regions and the phase of six in region II is critical for efficient RNA promoter activity. As expected, the gene start signal at the 3′ end of the NDV genome was required for mRNA transcription, but not for RNA replication. Surprisingly, mutation of the polyadenylation signal in the 5′ end did not affect gene expression transcription. Although the conserved region I of NDV (avulavirus) promoter appears to be more similar to that of Sendai virus (SeV) (respirovirus), conserved region II is analogous to that of rubulaviruses.",

keywords = "Newcastle disease virus, Paramyxovirus, RNA promoter, RNA replication, RNA transcription",

author = "Fernando Marcos and Laura Ferreira and Jerome Cros and Park, {Man Seong} and Takaaki Nakaya and Adolfo Garc{\'i}a-Sastre and Enrique Villar",

note = "Funding Information: This work was partially supported by the Spanish Fondo de Investigaciones Sanitarias FIS-PI021848 (cofinanced by the European Union FEDER funds within the FEDER-FSE 2000-2006 framework) and Junta de Castilla y Le{\'o}n (SA064/02) grants to E.V. and NIH grants to AG-S; F.M. was a fellowship holder from Junta de Castilla y Le{\'o}n. The vacT7 was provided by Dr. E. Berger and the BSRT7 cells were provided by Dr. M. Schnell. Thanks are also due to Dr. Griff Parks for his critical discussions.",

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AU - Marcos, Fernando

AU - Ferreira, Laura

AU - Cros, Jerome

AU - Park, Man Seong

AU - Nakaya, Takaaki

AU - García-Sastre, Adolfo

AU - Villar, Enrique

N1 - Funding Information: This work was partially supported by the Spanish Fondo de Investigaciones Sanitarias FIS-PI021848 (cofinanced by the European Union FEDER funds within the FEDER-FSE 2000-2006 framework) and Junta de Castilla y León (SA064/02) grants to E.V. and NIH grants to AG-S; F.M. was a fellowship holder from Junta de Castilla y León. The vacT7 was provided by Dr. E. Berger and the BSRT7 cells were provided by Dr. M. Schnell. Thanks are also due to Dr. Griff Parks for his critical discussions.

PY - 2005/1/20

Y1 - 2005/1/20

N2 - The RNA promoters of the genome and antigenome of Newcastle disease virus (NDV) were studied by mutational analysis of their 3′ terminal ends. Similarly to other paramyxoviruses, NDV RNA replication follows the rule of six, and the genomic and antigenomic promoters require two discontinuous regions: conserved region I (first 18 nucleotides) and conserved region II (nucleotides 73-90). Proper spacing between those regions and the phase of six in region II is critical for efficient RNA promoter activity. As expected, the gene start signal at the 3′ end of the NDV genome was required for mRNA transcription, but not for RNA replication. Surprisingly, mutation of the polyadenylation signal in the 5′ end did not affect gene expression transcription. Although the conserved region I of NDV (avulavirus) promoter appears to be more similar to that of Sendai virus (SeV) (respirovirus), conserved region II is analogous to that of rubulaviruses.

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Mapping of the RNA promoter of Newcastle disease virus

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