Mapping of the RNA promoter of Newcastle disease virus

Fernando Marcos, Laura Ferreira, Jerome Cros, Man-Seong Park, Takaaki Nakaya, Adolfo García-Sastre, Enrique Villar

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The RNA promoters of the genome and antigenome of Newcastle disease virus (NDV) were studied by mutational analysis of their 3′ terminal ends. Similarly to other paramyxoviruses, NDV RNA replication follows the rule of six, and the genomic and antigenomic promoters require two discontinuous regions: conserved region I (first 18 nucleotides) and conserved region II (nucleotides 73-90). Proper spacing between those regions and the phase of six in region II is critical for efficient RNA promoter activity. As expected, the gene start signal at the 3′ end of the NDV genome was required for mRNA transcription, but not for RNA replication. Surprisingly, mutation of the polyadenylation signal in the 5′ end did not affect gene expression transcription. Although the conserved region I of NDV (avulavirus) promoter appears to be more similar to that of Sendai virus (SeV) (respirovirus), conserved region II is analogous to that of rubulaviruses.

Original languageEnglish
Pages (from-to)396-406
Number of pages11
JournalVirology
Volume331
Issue number2
DOIs
Publication statusPublished - 2005 Jan 20
Externally publishedYes

Fingerprint

Newcastle disease virus
RNA
Respirovirus
Rubulavirus
Avulavirus
Nucleotides
Genome
Sendai virus
Polyadenylation
Virus Replication
Gene Expression
Messenger RNA
Mutation
Genes

Keywords

  • Newcastle disease virus
  • Paramyxovirus
  • RNA promoter
  • RNA replication
  • RNA transcription

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Marcos, F., Ferreira, L., Cros, J., Park, M-S., Nakaya, T., García-Sastre, A., & Villar, E. (2005). Mapping of the RNA promoter of Newcastle disease virus. Virology, 331(2), 396-406. https://doi.org/10.1016/j.virol.2004.10.040

Mapping of the RNA promoter of Newcastle disease virus. / Marcos, Fernando; Ferreira, Laura; Cros, Jerome; Park, Man-Seong; Nakaya, Takaaki; García-Sastre, Adolfo; Villar, Enrique.

In: Virology, Vol. 331, No. 2, 20.01.2005, p. 396-406.

Research output: Contribution to journalArticle

Marcos, F, Ferreira, L, Cros, J, Park, M-S, Nakaya, T, García-Sastre, A & Villar, E 2005, 'Mapping of the RNA promoter of Newcastle disease virus', Virology, vol. 331, no. 2, pp. 396-406. https://doi.org/10.1016/j.virol.2004.10.040
Marcos F, Ferreira L, Cros J, Park M-S, Nakaya T, García-Sastre A et al. Mapping of the RNA promoter of Newcastle disease virus. Virology. 2005 Jan 20;331(2):396-406. https://doi.org/10.1016/j.virol.2004.10.040
Marcos, Fernando ; Ferreira, Laura ; Cros, Jerome ; Park, Man-Seong ; Nakaya, Takaaki ; García-Sastre, Adolfo ; Villar, Enrique. / Mapping of the RNA promoter of Newcastle disease virus. In: Virology. 2005 ; Vol. 331, No. 2. pp. 396-406.
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