Mapping RNA regions in eukaryotic ribosomes that are accessible to methidiumpropyl-EDTA·Fe(II) and EDTA·Fe(II)

Hogyu Han, Alanna Schepartz, Maria Pellegrini, Peter B. Dervan

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Methidiumpropyl-EDTA·Fe(II) [MPE·Fe(II)] and EDTA·Fe(II) were used to investigate the structure of Drosophila melanogaster ribosomes. Cleavage reactions were performed on intact ribosomes in cell lysates in vitro and analyzed by primer extension with reverse transcriptase using oligodeoxynucleotide primers. Regions of 18S and 28S ribosomal RNAs (rRNAs) which are accessible to MPE·Fe(II) and EDTA·Fe(II) are located almost exclusively within expansion segments. The accessibility of these regions to cleavage indicates that they are likely exposed on the surface of eukaryotic ribosomes. These results provide information about the overall tertiary structure of rRNA in ribosomes.

Original languageEnglish
Pages (from-to)9831-9844
Number of pages14
JournalBiochemistry
Volume33
Issue number33
Publication statusPublished - 1994 Dec 1
Externally publishedYes

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Ribosomal RNA
Oligodeoxyribonucleotides
RNA-Directed DNA Polymerase
Ribosomes
Drosophila melanogaster

ASJC Scopus subject areas

  • Biochemistry

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Mapping RNA regions in eukaryotic ribosomes that are accessible to methidiumpropyl-EDTA·Fe(II) and EDTA·Fe(II). / Han, Hogyu; Schepartz, Alanna; Pellegrini, Maria; Dervan, Peter B.

In: Biochemistry, Vol. 33, No. 33, 01.12.1994, p. 9831-9844.

Research output: Contribution to journalArticle

Han, Hogyu ; Schepartz, Alanna ; Pellegrini, Maria ; Dervan, Peter B. / Mapping RNA regions in eukaryotic ribosomes that are accessible to methidiumpropyl-EDTA·Fe(II) and EDTA·Fe(II). In: Biochemistry. 1994 ; Vol. 33, No. 33. pp. 9831-9844.
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