Mapping RNA Regions in Eukaryotic Ribosomes That Are Accessible to Methidiumpropyl-EDTA·Fe(II) and EDTA·FeII)

Hogyu Han; Alanna Schepartz; Peter B. Dervan; Maria Pellegrini

doi:10.1021/bi00199a004

Mapping RNA Regions in Eukaryotic Ribosomes That Are Accessible to Methidiumpropyl-EDTA·Fe(II) and EDTA·FeII)

Hogyu Han, Alanna Schepartz, Peter B. Dervan, Maria Pellegrini

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Methidiumpropyl-EDTA·Fe(II) [MPE·Fe(II)] and EDTA·Fe(II) were used to investigate the structure of Drosophila melanogaster ribosomes. Cleavage reactions were performed on intact ribosomes in cell lysates in vitro and analyzed by primer extension with reverse transcriptase using oligodeoxynucleotide primers. Regions of 18S and 28S ribosomal RNAs (rRNAs) which are accessible to MPE·Fe(II) and EDTA·Fe(II) are located almost exclusively within expansion segments. The accessibility of these regions to cleavage indicates that they are likely exposed on the surface of eukaryotic ribosomes. These results provide information about the overall tertiary structure of rRNA in ribosomes.

Original language	English
Pages (from-to)	9831-9844
Number of pages	14
Journal	Biochemistry
Volume	33
Issue number	33
DOIs	https://doi.org/10.1021/bi00199a004
Publication status	Published - 1994 Aug 1
Externally published	Yes

ASJC Scopus subject areas

Biochemistry

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title = "Mapping RNA Regions in Eukaryotic Ribosomes That Are Accessible to Methidiumpropyl-EDTA·Fe(II) and EDTA·FeII)",

abstract = "Methidiumpropyl-EDTA·Fe(II) [MPE·Fe(II)] and EDTA·Fe(II) were used to investigate the structure of Drosophila melanogaster ribosomes. Cleavage reactions were performed on intact ribosomes in cell lysates in vitro and analyzed by primer extension with reverse transcriptase using oligodeoxynucleotide primers. Regions of 18S and 28S ribosomal RNAs (rRNAs) which are accessible to MPE·Fe(II) and EDTA·Fe(II) are located almost exclusively within expansion segments. The accessibility of these regions to cleavage indicates that they are likely exposed on the surface of eukaryotic ribosomes. These results provide information about the overall tertiary structure of rRNA in ribosomes.",

author = "Hogyu Han and Alanna Schepartz and Dervan, {Peter B.} and Maria Pellegrini",

year = "1994",

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journal = "Biochemistry",

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T1 - Mapping RNA Regions in Eukaryotic Ribosomes That Are Accessible to Methidiumpropyl-EDTA·Fe(II) and EDTA·FeII)

AU - Han, Hogyu

AU - Schepartz, Alanna

AU - Dervan, Peter B.

AU - Pellegrini, Maria

PY - 1994/8/1

Y1 - 1994/8/1

N2 - Methidiumpropyl-EDTA·Fe(II) [MPE·Fe(II)] and EDTA·Fe(II) were used to investigate the structure of Drosophila melanogaster ribosomes. Cleavage reactions were performed on intact ribosomes in cell lysates in vitro and analyzed by primer extension with reverse transcriptase using oligodeoxynucleotide primers. Regions of 18S and 28S ribosomal RNAs (rRNAs) which are accessible to MPE·Fe(II) and EDTA·Fe(II) are located almost exclusively within expansion segments. The accessibility of these regions to cleavage indicates that they are likely exposed on the surface of eukaryotic ribosomes. These results provide information about the overall tertiary structure of rRNA in ribosomes.

AB - Methidiumpropyl-EDTA·Fe(II) [MPE·Fe(II)] and EDTA·Fe(II) were used to investigate the structure of Drosophila melanogaster ribosomes. Cleavage reactions were performed on intact ribosomes in cell lysates in vitro and analyzed by primer extension with reverse transcriptase using oligodeoxynucleotide primers. Regions of 18S and 28S ribosomal RNAs (rRNAs) which are accessible to MPE·Fe(II) and EDTA·Fe(II) are located almost exclusively within expansion segments. The accessibility of these regions to cleavage indicates that they are likely exposed on the surface of eukaryotic ribosomes. These results provide information about the overall tertiary structure of rRNA in ribosomes.

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SP - 9831

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JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

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Mapping RNA Regions in Eukaryotic Ribosomes That Are Accessible to Methidiumpropyl-EDTA·Fe(II) and EDTA·FeII)

Abstract

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