Abstract
Methidiumpropyl-EDTA·Fe(II) [MPE·Fe(II)] and EDTA·Fe(II) were used to investigate the structure of Drosophila melanogaster ribosomes. Cleavage reactions were performed on intact ribosomes in cell lysates in vitro and analyzed by primer extension with reverse transcriptase using oligodeoxynucleotide primers. Regions of 18S and 28S ribosomal RNAs (rRNAs) which are accessible to MPE·Fe(II) and EDTA·Fe(II) are located almost exclusively within expansion segments. The accessibility of these regions to cleavage indicates that they are likely exposed on the surface of eukaryotic ribosomes. These results provide information about the overall tertiary structure of rRNA in ribosomes.
| Original language | English |
|---|---|
| Pages (from-to) | 9831-9844 |
| Number of pages | 14 |
| Journal | Biochemistry |
| Volume | 33 |
| Issue number | 33 |
| Publication status | Published - 1994 Dec 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
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Mapping RNA regions in eukaryotic ribosomes that are accessible to methidiumpropyl-EDTA·Fe(II) and EDTA·Fe(II). / Han, Hogyu; Schepartz, Alanna; Pellegrini, Maria; Dervan, Peter B.
In: Biochemistry, Vol. 33, No. 33, 01.12.1994, p. 9831-9844.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mapping RNA regions in eukaryotic ribosomes that are accessible to methidiumpropyl-EDTA·Fe(II) and EDTA·Fe(II)
AU - Han, Hogyu
AU - Schepartz, Alanna
AU - Pellegrini, Maria
AU - Dervan, Peter B.
PY - 1994/12/1
Y1 - 1994/12/1
N2 - Methidiumpropyl-EDTA·Fe(II) [MPE·Fe(II)] and EDTA·Fe(II) were used to investigate the structure of Drosophila melanogaster ribosomes. Cleavage reactions were performed on intact ribosomes in cell lysates in vitro and analyzed by primer extension with reverse transcriptase using oligodeoxynucleotide primers. Regions of 18S and 28S ribosomal RNAs (rRNAs) which are accessible to MPE·Fe(II) and EDTA·Fe(II) are located almost exclusively within expansion segments. The accessibility of these regions to cleavage indicates that they are likely exposed on the surface of eukaryotic ribosomes. These results provide information about the overall tertiary structure of rRNA in ribosomes.
AB - Methidiumpropyl-EDTA·Fe(II) [MPE·Fe(II)] and EDTA·Fe(II) were used to investigate the structure of Drosophila melanogaster ribosomes. Cleavage reactions were performed on intact ribosomes in cell lysates in vitro and analyzed by primer extension with reverse transcriptase using oligodeoxynucleotide primers. Regions of 18S and 28S ribosomal RNAs (rRNAs) which are accessible to MPE·Fe(II) and EDTA·Fe(II) are located almost exclusively within expansion segments. The accessibility of these regions to cleavage indicates that they are likely exposed on the surface of eukaryotic ribosomes. These results provide information about the overall tertiary structure of rRNA in ribosomes.
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UR - http://www.scopus.com/inward/citedby.url?scp=0027981974&partnerID=8YFLogxK
M3 - Article
C2 - 8060991
AN - SCOPUS:0027981974
VL - 33
SP - 9831
EP - 9844
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 33
ER -