A Gram-staining-negative, aerobic, cream-coloured, marine bacterium, with rod-shaped cells, designated strain YJ-S3-2T, was isolated from salt flat sediment of Yongyu-do, Republic of Korea. YJ-S3-2T grew at pH 5.0–9.0 (optimum pH 7.0), 4–45 °C (optimum 30 °C) and with 1–18% (w/v) NaCl (optimum 6%). The results of 16S rRNA gene sequence analysis indicated that YJ-S3-2T was closely related to Marinobacter segnicrescens SS011B1-4T (97.0%) followed by, 'Marinobacter nanhaiticus' D15-8W (96.7%), Marinobacter bryozoorum 50-11T (96.7%), Marinobacter koreensis DSMZ 179240T T (96.5%) and Marinobacter bohaiensis T17T (96.5%). The average nucleotide identity (ANI) and the genome to genome distance calculator (GGDC) estimate values between YJ-S3-2T and related type strains were 73.7–79.8 and 19.9–22.5%, and also 73.5 and 20.7% with Marinobacter hydro-carbonoclasticus. YJ-S3-2T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10%) were C16:0 (22.3%), summed feature 9 (C17:1 iso ω9c/C16:0 10-methyl, 13.8%) and 3 (C16:1 ω7c/C16:1 ω6c, 11.9%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and two unidentified phospholipids. The DNA G+C content of YJ-S3-2T is 60.9 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, YJ-S3-2T should be classified as representing a novel species within the genus Marinobacter, for which name Marinobacter halodurans sp. nov. is proposed, with the type strain YJ-S3-2T (=KACC 19883T=KCTC 62937T=JCM 33109T).
|Number of pages||7|
|Journal||International Journal of Systematic and Evolutionary Microbiology|
|Publication status||Published - 2020|
- Halophilic bacteria
- Salt flat
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics