Mass spectrometry-based proteomics of single cells and organoids: The new generation of cancer research

P. V. Migisha Ntwali, Chae Eun Heo, Jong Yoon Han, Soo Yeon Chae, Minji Kim, Hung M. Vu, Min Sik Kim, Hugh I. Kim

Research output: Contribution to journalReview articlepeer-review

Abstract

Mass spectrometry (MS)-based proteomics is becoming a widely used technique for proteome characterization. Remarkable improvements in the resolution and sensitivity of MS instruments have enabled MS to address an ever-increasing array of biological questions. Nevertheless, the intrinsic cellular heterogeneity of biological systems continues to be a hurdle in MS-based proteomics for cancer research. MS-based proteomics of 3D cellular models and single-cell systems are being used as an effective analysis technique to characterize cellular heterogeneity among cancer cells. Proteomic analysis of 3D cellular models and single-cell systems provides a correlation between cellular heterogeneity and patient-specific responses to chemotherapy drugs, leading to effective and less toxic cancer treatments. Here, we review the pioneering achievements in MS-based proteomic analysis of 3D cell models and single-cell systems. Additionally, we provide insights into the contribution of MS-based proteomics, from examining tumoroid and single-cell systems to the development of precision medicine for cancer treatment.

Original languageEnglish
Article number116005
JournalTrAC - Trends in Analytical Chemistry
Volume130
DOIs
Publication statusPublished - 2020 Sep

Keywords

  • 3D cell
  • Cellular heterogeneity
  • Mass spectrometry (MS)
  • Proteomics
  • Single cell

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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