Measurement of blood coagulation with considering RBC aggregation through a microchip-based light transmission aggregometer

Hyunjung Lim, Jeonghun Nam, Shubin Xue, Sehyun Shin

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Even though blood coagulation can be tested by various methods and techniques, the effect of RBC aggregation on blood coagulation is not fully understood. The present study monitored clot formation in a microchip-based light transmission aggregometer. Citrated blood samples with and without the addition of calcium ion solution were initially disaggregated by rotating a stirrer in the microchip. After abrupt stop of the rotating stirrer, the transmitted light intensity over time was recorded. The syllectogram (light intensity vs. time graph) manifested a rapid increase that is associated with RBC aggregation followed by a decrease that is associated with blood coagulation. The time to reach the peak point was used as a new index of coagulation time (CT) and ranged from 200 to 500 seconds in the present measurements. The CT was inversely proportional to the concentration of fibrinogen, which enhances RBC aggregation. In addition, the CT was inversely proportional to the hematocrit, which is similar to the case of the prothrombin time (PT), as measured by a commercial coagulometer. Thus, we carefully concluded that RBC aggregation should be considered in tests of blood coagulation.

Original languageEnglish
Pages (from-to)211-218
Number of pages8
JournalClinical Hemorheology and Microcirculation
Volume47
Issue number3
DOIs
Publication statusPublished - 2011

Keywords

  • Coagulation
  • Fibrinogen
  • Hematocrit
  • RBC aggregation

ASJC Scopus subject areas

  • Physiology
  • Hematology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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