Measurement of MMP activity in synovial fluid in cases of osteoarthritis and acute inflammatory conditions of the knee joints using a fluorogenic peptide probe-immobilized diagnostic kit

Ju Hee Ryu, Aeju Lee, Myung Sook Huh, Junuk Chu, Kwang Meyung Kim, Byung Soo Kim, Kuiwon Choi, Ick Chan Kwon, Jong Woong Park, Inchan Youn

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Purpose: A fluorogenic peptide probe-immobilized diagnostic kit was used to analyze MMP activity in the synovial fluids (SFs) from patients with osteoarthritis (OA) and acute inflammatory conditions of the knee joint. Methods: The MMP diagnostic kit containing a polymer-conjugated MMP probe immobilized on a 96-well plate was utilized for high-throughput screening of MMP activity in SFs from OA patients (n = 33) and patients with acute inflammatory conditions of the knee joint (n = 5). Results: Compared to SF from OA patients, SF from patients with acute inflammatory conditions of the knee joint presented stronger NIR fluorescent signals. In gelatin zymography, most samples from patients with acute inflammatory conditions of the knee joint also displayed 92 kDa (pro-form) MMP-9 and faint 84 kDa (active form) MMP-9, while SF from OA patients did not display detectable MMP-9 activity. Conclusion: The presence of a strong fluorescence signal from the MMP diagnostic kit corresponded well with patients with acute inflammatory conditions of the knee joint. The results suggest that our MMP diagnostic kit can be useful in differentiation between early stages of OA and acute inflammatory conditions of the knee joint.

Original languageEnglish
Pages (from-to)198-206
Number of pages9
JournalTheranostics
Volume2
Issue number2
DOIs
Publication statusPublished - 2012 Apr 19

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Synovial Fluid
Knee Joint
Matrix Metalloproteinases
Osteoarthritis
Peptides
Gelatin
Polymers
Fluorescence

Keywords

  • Acute inflammatory conditions of the knee joint
  • Diagnostic kit
  • Matrix metalloproteinases
  • Osteoarthritis
  • Synovial fluid

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

Cite this

Measurement of MMP activity in synovial fluid in cases of osteoarthritis and acute inflammatory conditions of the knee joints using a fluorogenic peptide probe-immobilized diagnostic kit. / Ryu, Ju Hee; Lee, Aeju; Huh, Myung Sook; Chu, Junuk; Kim, Kwang Meyung; Kim, Byung Soo; Choi, Kuiwon; Kwon, Ick Chan; Park, Jong Woong; Youn, Inchan.

In: Theranostics, Vol. 2, No. 2, 19.04.2012, p. 198-206.

Research output: Contribution to journalArticle

Ryu, Ju Hee ; Lee, Aeju ; Huh, Myung Sook ; Chu, Junuk ; Kim, Kwang Meyung ; Kim, Byung Soo ; Choi, Kuiwon ; Kwon, Ick Chan ; Park, Jong Woong ; Youn, Inchan. / Measurement of MMP activity in synovial fluid in cases of osteoarthritis and acute inflammatory conditions of the knee joints using a fluorogenic peptide probe-immobilized diagnostic kit. In: Theranostics. 2012 ; Vol. 2, No. 2. pp. 198-206.
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