Measurement of naturally acquired humoral immune responses against the C-terminal region of the Plasmodium vivax MSP1 protein using protein arrays

Jun Hu Chen, Yue Wang, Kwon Soo Ha, Feng Lu, In Bum Suh, Chae Seung Lim, Jeong Hyun Park, Satoru Takeo, Takafumi Tsuboi, Eun Taek Han

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Protein arrays are powerful tools for antibody profiling and vaccine development against infectious agents. In the previous report, we successfully applied an antibody-based protein array for immunoprofiling of Plasmodium vivax infection. Herein, we developed a Ni-NTA surface based protein array to detect immune responses against the recombinant C-terminal region (19 and 42 kDa) of the P. vivax merozoite surface protein 1 (PvMSP1-19 and -42) from sera of vivax malaria patients. The PvMSP1-19 arrays detected P. vivax in 112 of 130 (86.2%; 95% CI, 83.2-89.2%) microscopically positive samples and 2 false positives were obtained among 100 sera samples from healthy subjects (2.0%; 95% CI, 0.6-3.4%). These results were in concordance with results of enzyme-linked immunosorbent assays (ELISA). Kappa values represented excellent agreement for the recombinant PvMSP1-19 protein against sera samples as measured by protein arrays and ELISA (Kappa=0.904, 95% CI: 0.849-0.960). The PvMSP1-42 protein arrays detected antibody response in 100 of 130 microscopically positive samples (76.9%; 95% CI, 72.4-86.8%) and 8 false positives were obtained in 100 healthy subjects (8.0%; 95% CI, 2.7-13.3%). There is no significant difference between the fluorescent intensity of antibody response to PvMSP1-19 and PvMSP1-42 in the positive sera samples (P>0.05). The novel protein array platform may be used for profiling naturally acquired humoral immune responses to P. vivax infection.

Original languageEnglish
Pages (from-to)1259-1266
Number of pages8
JournalParasitology Research
Volume109
Issue number5
DOIs
Publication statusPublished - 2011 Nov 1

Fingerprint

Merozoite Surface Protein 1
Plasmodium vivax
Protein Array Analysis
Humoral Immunity
humoral immunity
Proteins
antibodies
proteins
Malaria
Antibody Formation
Healthy Volunteers
Serum
Enzyme-Linked Immunosorbent Assay
Vivax Malaria
sampling
enzyme-linked immunosorbent assay
Antibodies
merozoites
surface proteins
vaccine development

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Measurement of naturally acquired humoral immune responses against the C-terminal region of the Plasmodium vivax MSP1 protein using protein arrays. / Chen, Jun Hu; Wang, Yue; Ha, Kwon Soo; Lu, Feng; Suh, In Bum; Lim, Chae Seung; Park, Jeong Hyun; Takeo, Satoru; Tsuboi, Takafumi; Han, Eun Taek.

In: Parasitology Research, Vol. 109, No. 5, 01.11.2011, p. 1259-1266.

Research output: Contribution to journalArticle

Chen, Jun Hu ; Wang, Yue ; Ha, Kwon Soo ; Lu, Feng ; Suh, In Bum ; Lim, Chae Seung ; Park, Jeong Hyun ; Takeo, Satoru ; Tsuboi, Takafumi ; Han, Eun Taek. / Measurement of naturally acquired humoral immune responses against the C-terminal region of the Plasmodium vivax MSP1 protein using protein arrays. In: Parasitology Research. 2011 ; Vol. 109, No. 5. pp. 1259-1266.
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